| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MBOAT2 |
| Uniprot No | Q6ZWT7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-350aa |
| 活性数据 | MPSLLEYLSYNCNFMGILAGPLCSYKDYITFIEGRSYHITQSGENGKEETQYERTEPSPNTAVVQKLLVCGLSLLFHLTICTTLPVEYNIDEHFQATASWPTKIIYLYISLLAARPKYYFAWTLADAINNAAGFGFRGYDENGAARWDLISNLRIQQIEMSTSFKMFLDNWNIQTALWLKRVCYERTSFSPTIQTFILSAIWHGVYPGYYLTFLTGVLMTLAARAMRNNFRHYFIEPSQLKLFYDVITWIVTQVAISYTVVPFVLLSIKPSLTFYSSWYYCLHILGILVLLLLPVKKTQRRKNTHENIQLSQSKKFDEGENSLGQNSFSTTNNVCNQNQEIASRHSSLKQ |
| 分子量 | 66.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4篇关于重组人MBOAT2蛋白的参考文献摘要概述：
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1. **文献名称**: *"Functional characterization of human MBOAT2 as a lysophospholipid acyltransferase"*
**作者**: Lee, J. et al.
**摘要**: 本研究在大肠杆菌系统中成功表达并纯化了重组人MBOAT2蛋白，发现其具有特异性催化溶血磷脂的酰基转移活性，提示其在细胞膜脂质重塑中的关键作用。实验证实其活性依赖特定的膜结合结构域。
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2. **文献名称**: *"Structural insights into the catalytic mechanism of MBOAT2 through cryo-EM analysis"*
**作者**: Zhang, Y. et al.
**摘要**: 通过冷冻电镜解析重组人MBOAT2蛋白的高分辨率结构，揭示了其酰基转移酶活性中心的构象变化，阐明了底物识别与催化机制，为针对脂代谢疾病的药物设计提供结构基础。
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3. **文献名称**: *"MBOAT2 knockout mice exhibit altered brain lipid profiles and cognitive deficits"*
**作者**: Smith, R.P. et al.
**摘要**: 利用重组MBOAT2蛋白生成特异性抗体，研究其在基因敲除小鼠中的表达缺失效应。发现MBOAT2缺失导致脑部脂质组成异常及认知功能障碍，提示其与神经退行性疾病的潜在关联。
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4. **文献名称**: *"High-throughput screening identifies MBOAT2 inhibitors as potential anticancer agents"*
**作者**: Chen, L. et al.
**摘要**: 基于重组人MBOAT2蛋白建立体外酶活体系，筛选出小分子抑制剂，并验证其通过干扰脂代谢抑制肿瘤细胞生长的效果，为癌症靶向治疗提供新方向。
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(注：上述文献为模拟示例，实际引用请通过学术数据库检索具体论文。)
Recombinant human MBOAT2 (Membrane Bound O-Acyltransferase Domain Containing 2) is a protein encoded by the *MBOAT2* gene, located on chromosome 2 in humans. As a member of the MBOAT enzyme family, it shares conserved structural features, including multiple transmembrane domains and a catalytic motif involved in acyltransferase activity. MBOAT2 is hypothesized to function as a lysophospholipid acyltransferase, participating in phospholipid remodeling by transferring acyl groups to lysophospholipids, which is critical for maintaining membrane composition and cellular signaling processes. However, its specific physiological substrates and precise biological roles remain under investigation.
The recombinant form of MBOAT2 is typically produced in heterologous expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications and membrane localization. It is often tagged (e.g., His-tag, FLAG-tag) for purification and detection. Studies using recombinant MBOAT2 aim to elucidate its enzymatic kinetics, substrate specificity, and interactions with lipids or proteins. Emerging evidence links MBOAT2 dysregulation to metabolic disorders, cancer, and neurological conditions, though mechanistic details are limited. Recombinant MBOAT2 serves as a vital tool for structural studies (e.g., cryo-EM) and drug discovery, particularly in targeting lipid metabolism pathways. Current research focuses on clarifying its role in lipid homeostasis and disease pathogenesis.
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