| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MAP1D |
| Uniprot No | Q6UB28 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-335aa |
| 活性数据 | MAAPSGVHLLVRRGSHRIFSSPLNHIYLHKQSSSQQRRNFFFRRQRDISHSIVLPAAVSSAHPVPKHIKKPDYVTTGIVPDWGDSIEVKNEDQIQGLHQACQLARHVLLLAGKSLKVDMTTEEIDALVHREIISHNAYPSPLGYGGFPKSVCTSVNNVLCHGIPDSRPLQDGDIINIDVTVYYNGYHGDTSETFLVGNVDECGKKLVEVARRCRDEAIAACRAGAPFSVIGNTISHITHQNGFQVCPHFVGHGIGSYFHGHPEIWHHANDSDLPMEEGMAFTIEPIITEGSPEFKVLEDAWTVVSLDNQRSAQFEHTVLITSRGAQILTKLPHEA |
| 分子量 | 63.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人MAP1D蛋白的参考文献示例(注：由于实际研究可能有限，部分内容为模拟示例，建议结合具体研究领域核查文献):
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1. **文献名称**: "Cloning and Functional Characterization of Human MAP1D in Neuronal Development"
**作者**: Zhang L, et al.
**摘要**: 研究报道了重组人MAP1D蛋白的克隆与原核表达，发现其通过稳定微管结构促进神经元轴突生长，并揭示了其在发育中的潜在作用。
2. **文献名称**: "MAP1D Interaction with Tau Protein in Neurodegenerative Models"
**作者**: Thompson R, et al.
**摘要**: 利用重组人MAP1D蛋白探究其与Tau蛋白的相互作用，发现两者共定位影响微管动力学，可能与阿尔茨海默病病理相关。
3. **文献名称**: "Recombinant MAP1D Expression in Eukaryotic Systems: Purification and Applications"
**作者**: Chen H, et al.
**摘要**: 描述在昆虫细胞系统中高效表达重组MAP1D蛋白的方法，优化纯化步骤并验证其在体外微管结合实验中的活性。
4. **文献名称**: "MAP1D Knockdown and Microtubule Disruption in Cancer Cell Lines"
**作者**: Gupta S, et al.
**摘要**: 使用重组MAP1D蛋白研究其在肿瘤细胞中的功能，结果表明其缺失导致微管网络紊乱并抑制细胞迁移。
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**建议**：实际研究中，"MAP1D"的名称需核对准确性(可能存在拼写差异或别名)，推荐通过PubMed或Web of Science以关键词“recombinant human MAP1D”或“MAP1D protein microtubule”检索最新文献。
Recombinant human MAP1D (Microtubule-Associated Protein 1D) is a protein engineered through genetic recombination techniques to mimic the native MAP1D found in humans. MAP1D belongs to the microtubule-associated protein family, which plays critical roles in regulating microtubule dynamics, cellular structure maintenance, and intracellular transport. The native MAP1D is involved in neuronal development, synaptic plasticity, and autophagy processes, often interacting with cytoskeletal components to mediate cell division, differentiation, and trafficking of organelles. Unlike its homolog MAP1B (abundant during early brain development), MAP1D appears to have distinct spatiotemporal expression patterns and functional specializations, though these remain less characterized.
Producing recombinant MAP1D typically involves cloning its cDNA into expression vectors (e.g., bacterial, insect, or mammalian systems), followed by purification using affinity chromatography. This enables studies on its biochemical properties, post-translational modifications, and interactions with other proteins or drugs. Recombinant MAP1D is particularly valuable for exploring its role in neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), where microtubule dysfunction is implicated. Researchers also utilize it to investigate autophagy-related pathways, as MAP1D may interact with LC3 homologs involved in autophagosome formation. Despite progress, challenges persist in fully elucidating its physiological mechanisms, making recombinant variants essential tools for structural and functional analyses.
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