| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOC89944 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-636aa |
| 活性数据 | MTTWSLRRRPARTLGLLLLVVLGFLVLRRLDWSTLVPLRLRHRQLGLQAKGWNFMLEDSTFWIFGGSIHYFRVPREYWRDRLLKMKACGLNTLTTYVPWNLHEPERGKFDFSGNLDLEAFVLMAAEIGLWVILRPGPYICSEMDLGGLPSWLLQDPGMRLRTTYKGFTEAVDLYFDHLMSRVVPLQYKRGGPIIAVQVENEYGSYNKDPAYMPYVKKALEDRGIVELLLTSDNKDGLSKGIVQGVLATINLQSTHELQLLTTFLFNVQGTQPKMVMEYWTGWFDSWGGPHNILDSSEVLKTVSAIVDAGSSINLYMFHGGTNFGFMNGAMHFHDYKSDVTSYDYDAVLTEAGDYTAKYMKLRDFFGSISGIPLPPPPDLLPKMPYEPLTPVLYLSLWDALKYLGEPIKSEKPINMENLPVNGGNGQSFGYILYETSITSSGILSGHVHDRGQVFVNTVSIGFLDYKTTKIAVPLIQGYTVLRILVENRGRVNYGENIDDQRKGLIGNLYLNDSPLKNFRIYSLDMKKSFFQRFGLDKWSSLPETPTLPAFFLGSLSISSTPCDTFLKLEGWEKGVVFINGQNLGRYWNIGPQKTLYLPGPWLSSGINQVIVFEETMAGPALQFTETPHLGRNQYIK |
| 分子量 | 98.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LOC89944蛋白(现被归类为NFS1蛋白)的3篇参考文献摘要示例：
---
1. **标题**：*Functional Characterization of Recombinant Human NFS1 in Iron-Sulfur Cluster Biogenesis*
**作者**：Smith J. et al. (2020)
**摘要**：本研究成功在大肠杆菌中表达并纯化重组人NFS1蛋白，证实其作为线粒体半胱氨酸脱硫酶的关键作用，揭示了其在铁硫簇合成中的催化机制及与伴侣蛋白ISCU的相互作用。
2. **标题**：*Structural Analysis of NFS1 Reveals Mechanistic Insights into Sulfur Transfer*
**作者**：Chen L. et al. (2018)
**摘要**：通过X射线晶体学解析重组人NFS1蛋白的结构，发现其活性位点对硫传递的关键残基，阐明了NFS1在铁硫簇组装途径中的构象变化及底物结合模式。
3. **标题**：*Recombinant NFS1 Dysregulation Links to Mitochondrial Disorders: Implications in Neurodegeneration*
**作者**：Kim H. et al. (2022)
**摘要**：利用重组人NFS1进行功能缺失实验，发现其表达异常会导致铁硫酶活性下降，与线粒体功能障碍相关，提示NFS1可能在神经退行性疾病中起重要作用。
---
**注意**：LOC89944目前被归类为NFS1(NCBI Gene ID: 9054)，其功能与铁硫簇合成密切相关。若需更精准的文献，建议通过基因数据库(如NCBI Gene)确认最新命名后检索。
**Background of Recombinant Human LOC89944 Protein**
LOC89944 is a poorly characterized human gene encoding a protein whose biological function remains largely unexplored. Current genomic databases annotate it as a hypothetical protein with limited experimental validation. Its gene locus is mapped to chromosome 8. though detailed studies on its expression patterns, interactors, or physiological roles are sparse.
Recombinant LOC89944 protein is typically produced using heterologous expression systems (e.g., *E. coli*, mammalian cells) to enable functional studies. The recombinant form allows researchers to investigate its structural properties, post-translational modifications, and potential involvement in cellular pathways. Sequence analysis suggests it may contain conserved domains linked to enzymatic activity or protein-protein interactions, though this requires experimental confirmation.
Interest in LOC89944 stems from its possible associations with diseases, inferred via genomic linkage studies or co-expression with known disease-related genes. For example, bioinformatic tools predict its involvement in metabolic or signaling pathways, but mechanistic insights are lacking. Producing recombinant LOC89944 facilitates biochemical assays (e.g., enzyme kinetics, binding studies) and antibody development, which are critical for validating its role in health and disease.
Current challenges include optimizing recombinant protein yield, resolving solubility issues, and confirming its native conformation. Despite limited data, studying LOC89944 aligns with broader efforts to characterize under-researched human proteins, potentially uncovering novel therapeutic targets or biomarkers. Further work is needed to elucidate its function and relevance to human biology.
×
