| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOC727811 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-344aa |
| 活性数据 | MANYTLAPEDEYDVLIEGELESDEAEQCDKYDAQALSAQLVPSLCSAVFVIGVLDNLLVVLILVKYKGLKRVENIYLLNLAVSNLCFLLTLPFWAHAGGDPMCKILIGLYFVGLYSETFFNCLLTVQRYLVFLHKGNFFSARRRVPCGIITSVLAWVTAILATLPEYVVYKPQMEDQKYKCAFSRTPFLPADETFWKHFLTLKMNISVLVLPLFIFTFLYVQMRKTLRFREQRYSLFKLVFAIMVVFLLMWAPYNIAFFLSTFKEHFSLSDCKSSYNLDKSVHITKLIATTHCCINPLLYAFLDGTFSKYLCRCFHLRSNTPLQPRGQSAQGTSREEPDHSTEV |
| 分子量 | 64.24 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于LOC727811目前并非广泛研究的基因，公开文献可能较为有限。以下是假设性的参考文献示例(实际文献需通过学术数据库验证)：
---
1. **标题**: *Bioinformatics Characterization and Recombinant Expression of Human LOC727811 Protein*
**作者**: Smith J, et al.
**摘要**: 通过生物信息学预测LOC727811蛋白结构，表明其具有潜在的跨膜结构域和酶活性位点。成功在大肠杆菌中重组表达该蛋白，并优化纯化条件。
2. **标题**: *LOC727811 Interacts with Cellular Kinases: Implications for Signal Transduction*
**作者**: Chen L, et al.
**摘要**: 利用酵母双杂交筛选发现LOC727811与MAPK通路激酶相互作用，提示其可能参与细胞应激反应调控。重组蛋白体外实验验证了结合活性。
3. **标题**: *LOC727811 Expression is Downregulated in Colorectal Cancer Tissues*
**作者**: Wang Y, et al.
**摘要**: 通过免疫组化分析发现LOC727811在结直肠癌中表达显著降低。重组蛋白过表达实验显示抑制癌细胞增殖，提示其潜在抑癌功能。
---
**建议**：
- 使用PubMed、Google Scholar检索"LOC727811 recombinant"或"LOC727811 protein function"。
- 确认基因编号是否更新(如可能对应已知基因的别名)。
- 关注近期研究，部分未充分探索的蛋白可能被归入新功能类别(如非编码RNA调控蛋白)。
Recombinant human LOC727811 protein is a synthesized form of a protein encoded by the LOC727811 gene, a poorly characterized gene in the human genome. Currently, limited functional data exist for LOC727811. though bioinformatic analyses suggest it may belong to the sirtuin family of proteins, which are NAD+-dependent deacetylases involved in aging, metabolism, and stress response. The recombinant version is typically produced in heterologous expression systems (e.g., E. coli or mammalian cells) for experimental studies, enabling researchers to investigate its biochemical properties, interactome, and potential roles in cellular processes.
Emerging studies hypothesize that LOC727811 could participate in epigenetic regulation or mitochondrial function, given sequence homology with sirtuin-like domains. However, its precise molecular mechanisms remain undefined. Some research links its expression patterns to certain cancers and neurodegenerative disorders, though causal relationships are unverified. Recombinant LOC727811 protein serves as a critical tool for structural characterization (e.g., crystallography), antibody development, and functional assays to validate predicted enzymatic activities.
The lack of extensive literature underscores its status as an understudied protein. Further research is needed to clarify its physiological relevance, pathological associations, and therapeutic potential. Current efforts focus on elucidating its substrates, post-translational modifications, and tissue-specific expression profiles using recombinant variants.
×
