| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LOC646074 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-235aa |
| 活性数据 | MNWLGAPSGGHIKNPQSEASLGLSDLGGICPGHLSFCFGPLSLGRWPDTWGWPKDASENLDSHSTDGPISQRFPGHLSASRRWTGTFRHRWRTGCSVSCCSGQGWPPAARSTAAPRPALAEWGRGEWLRGGLTAAGGEVFGAQVPKSTSGARNPCWCHEPLTVRRGWGGVLTRHDLLGGGWLFILEMHRALLCPPGWSTVAPSWITAASNSWAQAILLCQPPRYLGLQMCATMPD |
| 分子量 | 52.25 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于目前公开数据库中关于“重组人LOC646074蛋白”的研究有限，以下为基于类似蛋白研究的模拟参考文献范例(非真实存在)，供参考：
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1. **文献名称**: *LOC646074蛋白在细胞增殖调控中的作用机制*
**作者**: Zhang L., et al.
**摘要**: 本研究通过重组表达LOC646074蛋白，发现其与细胞周期蛋白依赖性激酶(CDK2)相互作用，并在体外抑制HeLa细胞的增殖。蛋白结构预测表明其含有一个新型螺旋-转角-螺旋结构域，可能参与DNA结合调控。
2. **文献名称**: *重组人LOC646074蛋白的晶体结构解析与功能预测*
**作者**: Kim S., et al.
**摘要**: 利用X射线晶体学首次解析了LOC646074蛋白的三维结构，揭示了其独特的β折叠核心结构，并推测其可能通过介导泛素化通路参与蛋白质降解调控，为癌症靶向治疗提供潜在靶点。
3. **文献名称**: *大肠杆菌表达系统中重组LOC646074蛋白的优化纯化及活性分析*
**作者**: Wang Y., et al.
**摘要**: 开发了一种高效的重组LOC646074蛋白表达和纯化方法，使用His标签系统在大肠杆菌中实现可溶性表达，并通过体外酶活实验证实该蛋白具有ATP酶活性，可能参与能量代谢应激反应。
4. **文献名称**: *LOC646074基因敲除小鼠模型中异常免疫应答的表型研究*
**作者**: Müller R., et al.
**摘要**: 通过构建LOC646074基因敲除小鼠，发现该蛋白缺失导致T细胞活化异常和自身免疫疾病表型，提示其在免疫调节中的关键作用，重组蛋白的补充实验可部分逆转表型。
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**注意**：以上文献为模拟示例，实际研究中请通过PubMed、Google Scholar等平台以“LOC646074”或“重组蛋白表达”等关键词检索最新成果。若研究较少，可扩展至该基因的同源蛋白或相关通路文献。
The human LOC646074 protein, encoded by the LOC646074 gene, is a poorly characterized protein with limited experimental data available in current scientific literature. Its gene is located on chromosome 19 (19q13.12) and is classified as a "long non-coding RNA" or a protein-coding gene depending on annotation databases, reflecting ongoing debates about its functional nature. Bioinformatic analyses suggest it may contain conserved structural domains, possibly involved in intracellular signaling or molecular interactions, though no specific mechanism has been validated.
As a recombinant protein, LOC646074 has drawn interest in proteomic studies and drug discovery pipelines due to its differential expression patterns observed in RNA-seq data from certain cancers and inflammatory conditions. Researchers produce recombinant LOC646074 using bacterial or mammalian expression systems to study its biochemical properties, potential binding partners, and post-translational modifications. However, its physiological role remains speculative, with hypotheses ranging from epigenetic regulation to stress-response pathways. Current research focuses on clarifying its subcellular localization, tissue-specific expression profiles, and possible associations with diseases like hepatocellular carcinoma or autoimmune disorders. The lack of antibodies and knockout models significantly limits functional studies, making recombinant protein tools crucial for advancing this understudied target.
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