| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCE4A |
| Uniprot No | Q5TA78 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-99aa |
| 活性数据 | MSCQQNQQQCQPPPKCPIPKYPPKCPSKCASSCPPPISSCCGSSSGGCGCCSSEGGGCCLSHHRHHRSHCHRPKSSNCYGSGSGQQSGGSGCCSGGGCC |
| 分子量 | 10.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为模拟生成的关于重组人LCE4A蛋白的参考文献示例(实际文献需通过学术数据库查询)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant Human LCE4A in Epidermal Keratinocytes*
**作者**：Smith J et al.
**摘要**：本研究利用哺乳动物表达系统成功表达并纯化了重组人LCE4A蛋白，证实其在体外促进角质细胞分化和表皮屏障相关蛋白(如loricrin)的表达，提示LCE4A可能在皮肤屏障功能中起关键作用。
2. **文献名称**：*LCE4A Gene Variants and Protein Dysregulation in Psoriasis Pathogenesis*
**作者**：Wang L et al.
**摘要**：通过分析银屑病患者皮肤样本，发现LCE4A表达水平显著下调。重组LCE4A蛋白的体外实验表明，其缺失可能导致角化异常和炎症因子(如IL-17)上调，为银屑病机制提供新见解。
3. **文献名称**：*Structural Analysis of Recombinant LCE4A Reveals Potential Protein-Protein Interaction Domains*
**作者**：Garcia R et al.
**摘要**：通过X射线晶体学解析了重组人LCE4A蛋白的三维结构，鉴定出其C端富含脯氨酸的结构域可能参与与其他角化包膜蛋白(如 involucrin)的相互作用，为研究表皮分化机制提供结构基础。
4. **文献名称**：*LCE4A Knockout Mice Exhibit Impaired Cutaneous Barrier and Enhanced Allergic Sensitization*
**作者**：Tanaka K et al.
**摘要**：构建LCE4A基因敲除小鼠模型，发现其表皮通透性增加且易诱发过敏性皮炎。外源性重组LCE4A蛋白局部应用可部分修复屏障缺陷，提示其治疗潜力。
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**提示**：以上内容为基于LCE蛋白家族已知功能的模拟文献概括，实际研究需查询PubMed、Web of Science等平台，关键词为“recombinant LCE4A”或“LCE4A protein function”。
Late Cornified Envelope 4A (LCE4A) is a member of the human late cornified envelope (LCE) protein family, which plays critical roles in epidermal differentiation and skin barrier formation. These proteins are primarily expressed during the terminal differentiation of keratinocytes, contributing to the structural integrity of the cornified envelope—a protective outer layer of the epidermis. LCE4A, encoded within the epidermal differentiation complex on chromosome 1q21. shares characteristic features with other LCE proteins, including a high content of glycine and serine residues that facilitate cross-linking in the stratum corneum.
Studies suggest LCE genes, including LCE4A, are associated with skin disorders such as psoriasis and atopic dermatitis. Genetic deletion of LCE cluster genes (LCE3B/LCE3C) has been linked to psoriasis susceptibility, highlighting their role in skin inflammation and repair. While LCE4A's exact mechanistic function remains under investigation, its expression is modulated by environmental stressors, infections, or immune signals, suggesting involvement in barrier maintenance and innate immune responses.
Recombinant human LCE4A protein is produced via heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Its recombinant form enables exploration of its interactions with epidermal lipids, enzymatic processes in cornification, and immunomodulatory properties. Research on LCE4A advances our understanding of epithelial pathologies and potential therapeutic strategies for chronic skin diseases.
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