| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCE1E |
| Uniprot No | Q5T753 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-118aa |
| 活性数据 | MSCQQSQQQCQPPPKCTPKCPPKCPTPKCPPKCPPKCPPVSSCCSVSSGGCCGSSSGGSCGSSSGGCCSSGGGGCCLSHHRHHRSHRHRPQSSDCCSQPSGGSSCCGGGSGQHSGGCC |
| 分子量 | 13 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LCE1E蛋白的模拟参考文献(注：部分内容可能为示例性描述，建议通过学术数据库核实具体文献)：
1. **文献名称**: *"Cloning and Expression of Recombinant Human LCE1E Protein in Escherichia coli"*
**作者**: Zhang Y, et al.
**摘要**: 研究报道了人LCE1E基因的克隆及其在大肠杆菌中的重组表达，通过优化诱导条件提高蛋白可溶性，并利用亲和层析纯化获得高纯度蛋白，为后续功能研究奠定基础。
2. **文献名称**: *"Structural Characterization of Recombinant LCE1E and Its Role in Skin Barrier Function"*
**作者**: Smith JL, et al.
**摘要**: 解析了重组人LCE1E蛋白的二级结构，证明其与表皮分化相关，并通过体外实验发现该蛋白可能通过调控角质形成细胞的增殖参与银屑病病理过程。
3. **文献名称**: *"Antimicrobial Activity of Recombinant LCE1E Protein in Psoriatic Epidermis"*
**作者**: Wang H, et al.
**摘要**: 利用重组LCE1E蛋白进行功能验证，发现其对金黄色葡萄球菌具有抑制作用，提示LCE1E在皮肤先天免疫防御中可能发挥重要作用。
4. **文献名称**: *"LCE1E Gene Polymorphisms and Protein Expression in Inflammatory Skin Diseases"*
**作者**: Gupta R, et al.
**摘要**: 通过构建重组LCE1E蛋白，分析其在不同皮肤炎症患者组织中的表达差异，探讨基因多态性与蛋白功能缺失的关联性。
**注意**：以上文献为示例性内容，实际研究中请通过**PubMed**或**Web of Science**以“LCE1E recombinant protein”等关键词检索最新成果。
Recombinant human LCE1E (Late Cornified Envelope 1E) protein is a genetically engineered variant of a naturally occurring protein belonging to the LCE protein family, which plays a critical role in epidermal differentiation and skin barrier formation. LCE1E is part of the late cornified envelope gene cluster located on chromosome 1q21. a region associated with epidermal homeostasis and inflammatory skin disorders like psoriasis. These proteins are predominantly expressed in the suprabasal layers of the epidermis, contributing to the assembly of the cornified envelope—a structure essential for maintaining skin integrity, hydration, and defense against environmental stressors.
The LCE1E gene, like other LCE family members, is regulated during keratinocyte differentiation and is thought to participate in cross-linking keratin intermediate filaments during cornification. Its dysfunction or altered expression has been implicated in psoriasis pathogenesis, as genome-wide studies have identified LCE gene deletions as risk factors. Recombinant LCE1E protein is typically produced using heterologous expression systems (e.g., E. coli or mammalian cells) for functional studies. Researchers utilize this protein to investigate its biochemical properties, interaction partners, and role in skin diseases, offering insights into therapeutic strategies for barrier defects or inflammatory dermatoses. Its applications extend to in vitro models of epidermal development and high-throughput screening for drug discovery.
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