| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCE2B |
| Uniprot No | O14633 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-110aa |
| 活性数据 | MSCQQNQQQCQPPPKCPPKCTPKCPPKCPPKCLPQCPAPCSPAVSSCCGPISGGCCGPSSGGCCNSGAGGCCLSHHRPRLFHRRRHQSPDCCESEPSGGSGCCHSSGGCC |
| 分子量 | 38.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于重组人LCE2B蛋白相关研究的**模拟参考文献示例**（实际文献需通过学术数据库验证）：
1. **标题**: "Expression and functional characterization of recombinant human LCE2B in keratinocyte differentiation"
**作者**: Zhang et al.
**摘要**: 研究通过大肠杆菌表达重组人LCE2B蛋白，验证其在体外促进角质形成细胞分化的功能，揭示其在表皮屏障修复中的潜在作用。
2. **标题**: "LCE2B gene variants and protein expression in psoriasis susceptibility"
**作者**: Smith et al.
**摘要**: 分析LCE2B基因多态性与银屑病易感性的关联，并通过重组蛋白实验证明LCE2B缺失导致表皮脂质代谢异常。
3. **标题**: "Recombinant LCE2B protein enhances skin permeability in an in vitro model"
**作者**: Wang et al.
**摘要**: 利用重组人LCE2B蛋白处理三维皮肤模型，发现其通过调节角化包膜蛋白交联影响皮肤渗透性，为药物递送研究提供新思路。
4. **标题**: "Optimization of recombinant human LCE2B production using a yeast expression system"
**作者**: Kim et al.
**摘要**: 探讨毕赤酵母系统中重组LCE2B的高效表达及纯化工艺，提高蛋白产量用于后续结构分析。
**注意**：以上内容为模拟生成，真实文献建议通过 **PubMed、Google Scholar** 或 **Web of Science** 检索关键词 "recombinant human LCE2B" 或 "LCE2B protein function"。
Recombinant human LCE2B (Late Cornified Envelope 2B) protein is a genetically engineered variant of the naturally occurring LCE2B protein, which belongs to the Late Cornified Envelope (LCE) gene family. These genes are clustered within the epidermal differentiation complex on chromosome 1q21. a region critical for skin barrier formation and maintenance. LCE2B is specifically expressed in the upper layers of the epidermis and plays a role in terminal keratinocyte differentiation, contributing to the assembly of the cornified envelope—a protein-lipid structure essential for skin integrity and hydration.
Mutations or altered expression of LCE genes, including LCE2B, have been associated with psoriasis, atopic dermatitis, and other skin disorders, highlighting its importance in epidermal homeostasis. The recombinant protein is typically produced using bacterial or mammalian expression systems, enabling studies on its molecular interactions, structural properties, and functional mechanisms. Researchers utilize recombinant LCE2B to investigate its role in skin barrier dysfunction, inflammatory responses, and wound healing. It also serves as a tool for developing therapeutic strategies targeting skin diseases or evaluating environmental stressors' effects on epidermal proteins. Despite its significance, challenges in solubility, proper folding, and post-translational modifications during recombinant production require optimization for functional studies.
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