| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCE3B |
| Uniprot No | Q5TA77 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-95aa |
| 活性数据 | MSCQQNQQQCQPLPKCPSPKCPPKSSAQCLPPASSCCAPRPGCCGGPSSEGGCCLSHHRCCRSHRCRRQSSNSCDRGSGQQDGASDCGYGSGGCC |
| 分子量 | 10.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人LCE3B蛋白的3篇代表性文献及其摘要概括：
1. **《LCE3B protein expression in psoriatic epidermis》**
*作者：Smith A, et al.*
摘要：研究揭示了LCE3B蛋白在银屑病患者皮肤中的异常表达，通过重组技术表达了人LCE3B蛋白，发现其参与皮肤屏障功能调控，可能与炎症因子IL-17的相互作用有关。
2. **《Recombinant human LCE3B: production in E. coli and structural characterization》**
*作者：Zhang Y, et al.*
摘要：首次报道利用大肠杆菌表达系统高效制备重组人LCE3B蛋白，并通过质谱和圆二色谱分析其结构，证明该蛋白具有典型的β-折叠构象，为后续功能研究提供基础。
3. **《LCE3B regulates keratinocyte differentiation through TLR3 signaling》**
*作者：Wang L, et al.*
摘要：研究发现重组LCE3B蛋白可通过激活TLR3通路促进角质形成细胞分化，且其功能缺失与特应性皮炎相关，提示其在皮肤免疫及修复中的作用。
4. **《Functional analysis of late cornified envelope (LCE) proteins in epidermal development》**
*作者：Jackson B, et al.*
摘要：通过重组LCE3B蛋白体外实验，证明其参与表皮终末分化过程，敲低LCE3B会导致角质层结构缺陷，证实其对皮肤屏障完整性的关键影响。
(注：以上文献为基于领域研究的模拟示例，实际引用时需查询具体论文。)
Recombinant human LCE3B (Late Cornified Envelope 3B) protein is a genetically engineered variant of the naturally occurring LCE3B protein, a member of the late cornified envelope (LCE) gene family. This family comprises over 40 genes clustered on chromosome 1q21. a region linked to epidermal differentiation and skin barrier function. LCE proteins are predominantly expressed in the epidermis, particularly during keratinocyte terminal differentiation, and contribute to the formation of the cornified envelope—a critical structure for skin integrity and hydration. LCE3B, specifically, is characterized by its cysteine-rich structure and potential involvement in cross-linking keratinocytes during cornification.
Interest in recombinant LCE3B stems from its association with inflammatory skin disorders. Genome-wide studies identified LCE3B deletions as susceptibility factors in psoriasis, suggesting its role in immune regulation or barrier repair. Recombinant LCE3B enables functional studies to dissect its molecular mechanisms in skin homeostasis and pathology. Produced via bacterial or mammalian expression systems, the purified protein is utilized to investigate interactions with lipids, antimicrobial peptides, or immune mediators. It also serves as an antigen in autoimmune studies, given the potential link between LCE proteins and psoriatic autoimmunity. Current research focuses on its therapeutic potential in epithelial regeneration, wound healing, and modulating inflammatory responses.
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