| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LCE2C |
| Uniprot No | Q5TA81 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-110aa |
| 活性数据 | MSCQQNQQQCQPPPKCPPKCTPKCPPKCPPKCPPQCPAPCFPAVSSCCGPSSGSCCGPSSGGCCSSGAGGCSLSHHRPRLFHRRRHQSPDCCESEPSGGSGCCHSSGGCC |
| 分子量 | 12.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的重组人LCE2C蛋白相关参考文献示例(注：文献信息为假设性示范，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：Expression and purification of recombinant human LCE2C protein in *E. coli* for functional studies
**作者**：Li, X., et al. (2015)
**摘要**：报道了通过大肠杆菌系统高效表达重组人LCE2C蛋白，采用His标签亲和层析纯化，并验证其促进角质形成细胞分化的功能，为研究LCE2C在皮肤屏障中的作用提供工具。
2. **文献名称**：Structural characterization of LCE2C reveals insights into its role in epidermal differentiation
**作者**：Chen, Y., et al. (2017)
**摘要**：通过核磁共振(NMR)解析LCE2C蛋白的溶液结构，发现其具有保守的α-螺旋结构域，可能通过蛋白-脂质相互作用参与表皮细胞终末分化调控。
3. **文献名称**：Dysregulation of LCE2C in psoriatic skin: Implications for disease pathogenesis
**作者**：Zhang, H., et al. (2018)
**摘要**：发现银屑病患者皮损中LCE2C表达显著下调，体外实验表明重组LCE2C蛋白可抑制促炎因子(如IL-17)的释放，提示其具有潜在免疫调节功能。
4. **文献名称**：Development of a keratinocyte-specific delivery system for recombinant LCE2C in atopic dermatitis models
**作者**：Wang, Q., et al. (2020)
**摘要**：构建了纳米颗粒包裹的重组LCE2C蛋白递送系统，在小鼠特应性皮炎模型中观察到皮肤屏障修复和炎症减轻的效果，为治疗策略提供新思路。
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**注意**：以上文献为示例性质，具体研究请通过 **PubMed/Google Scholar** 检索关键词 "recombinant human LCE2C" 或 "LCE2C protein function" 获取真实文献。
**Background of Recombinant Human LCE2C Protein**
Late Cornified Envelope (LCE) proteins are critical components of the epidermal differentiation complex, playing essential roles in skin barrier formation and maintenance. LCE2C, a member of the LCE2 subfamily, is a cysteine-rich structural protein predominantly expressed in differentiated keratinocytes. It contributes to the assembly of the cornified envelope, a resilient structure formed during terminal keratinocyte differentiation, which protects against environmental stressors and pathogens.
Genetically, the LCE gene cluster is located on chromosome 1q21. a region linked to inflammatory skin disorders like psoriasis. Variations in LCE genes, including LCE2C, are associated with altered susceptibility to psoriasis, suggesting its involvement in epidermal integrity and immune responses. However, the exact molecular mechanisms of LCE2C remain understudied compared to other LCE members.
Recombinant human LCE2C protein is produced via heterologous expression systems (e.g., E. coli or mammalian cells) to enable functional studies. Its purified form facilitates research on protein-protein interactions, epidermal barrier modeling, and pathological pathways in skin diseases. Current studies focus on elucidating its role in inflammation, keratinocyte differentiation, and potential therapeutic applications. Despite progress, further investigations are needed to clarify its distinct biological functions and clinical relevance.
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