| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | L3MBTL2 |
| Uniprot No | Q969R5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-705aa |
| 活性数据 | MEKPRSIEETPSSEPMEEEEDDDLELFGGYDSFRSYNSSVGSESSSYLEESSEAENEDREAGELPTSPLHLLSPGTPRSLDGSGSEPAVCEMCGIVGTREAFFSKTKRFCSVSCSRSYSSNSKKASILARLQGKPPTKKAKVLHKAAWSAKIGAFLHSQGTGQLADGTPTGQDALVLGFDWGKFLKDHSYKAAPVSCFKHVPLYDQWEDVMKGMKVEVLNSDAVLPSRVYWIASVIQTAGYRVLLRYEGFENDASHDFWCNLGTVDVHPIGWCAINSKILVPPRTIHAKFTDWKGYLMKRLVGSRTLPVDFHIKMVESMKYPFRQGMRLEVVDKSQVSRTRMAVVDTVIGGRLRLLYEDGDSDDDFWCHMWSPLIHPVGWSRRVGHGIKMSERRSDMAHHPTFRKIYCDAVPYLFKKVRAVYTEGGWFEEGMKLEAIDPLNLGNICVATVCKVLLDGYLMICVDGGPSTDGLDWFCYHASSHAIFPATFCQKNDIELTPPKGYEAQTFNWENYLEKTKSKAAPSRLFNMDCPNHGFKVGMKLEAVDLMEPRLICVATVKRVVHRLLSIHFDGWDSEYDQWVDCESPDIYPVGWCELTGYQLQPPVAAEPATPLKAKEATKKKKKQFGKKRKRIPPTKTRPLRQGSKKPLLEDDPQGARKISSEPVPGEIIAVRVKEEHLDVASPDKASSPELPVSVENIKQETDD |
| 分子量 | 103.95 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人L3MBTL2蛋白研究的文献示例(部分为虚构，仅供参考格式)：
1. **文献名称**：*"Structural insights into the methyl-binding activity of human L3MBTL2 protein"*
**作者**：Smith A, et al.
**摘要**：本研究通过重组表达人L3MBTL2蛋白，解析了其MBT结构域结合组蛋白H4K20单甲基化的分子机制，揭示了其参与染色质修饰和基因沉默的功能基础。
2. **文献名称**：*"L3MBTL2 interacts with Polycomb repressive complex 1 and regulates cellular proliferation"*
**作者**：Chen L, et al.
**摘要**：利用重组人L3MBTL2蛋白进行互作实验，发现其通过与Polycomb复合物协同作用，抑制靶基因转录，在肿瘤细胞中发挥增殖调控作用。
3. **文献名称**：*"Recombinant L3MBTL2 suppresses Wnt/β-catenin signaling by binding to Hmgb1 in vitro"*
**作者**：Wang Y, et al.
**摘要**：通过大肠杆菌系统表达重组人L3MBTL2蛋白，证明其直接结合Hmgb1并拮抗Wnt通路活性，为开发癌症治疗靶点提供依据。
**注**：以上文献为示例性内容，实际引用时请通过PubMed或Web of Science检索真实论文。建议关注关键词：**L3MBTL2 recombination, MBT domain, chromatin regulation, cancer signaling**。
**Background of Recombinant Human L3MBTL2 Protein**
The human L3MBTL2 (lethal(3)malignant brain tumor-like 2) protein is a member of the Polycomb group (PcG) protein family, which plays critical roles in epigenetic regulation, particularly in chromatin compaction and transcriptional repression. It contains multiple MBT (malignant brain tumor) domains that mediate binding to methylated lysine residues on histones, such as H4K20me1/2 and H1bK26me1/2. linking histone modifications to chromatin structure modulation. L3MBTL2 functions as a chromatin reader, facilitating the formation of transcriptionally repressive complexes.
Recombinant L3MBTL2 is produced via molecular cloning and expression in heterologous systems (e.g., *E. coli* or mammalian cell lines) for biochemical and functional studies. Its recombinant form enables researchers to explore mechanisms underlying gene silencing, cell cycle regulation, and DNA repair. Studies highlight its role in antagonizing oncogenic pathways by recruiting repressive complexes, such as the Polycomb repressive complex 1 (PRC1), or collaborating with other tumor suppressors like p53. Dysregulation of L3MBTL2 is implicated in malignancies, including hematological cancers, where its loss or mutation may disrupt epigenetic homeostasis.
As a research tool, recombinant L3MBTL2 aids in dissecting molecular interactions in chromatin biology, offering insights into therapeutic strategies targeting epigenetic dysregulation in diseases.
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