| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KSR |
| Uniprot No | Q8IVT5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-762aa |
| 活性数据 | MNEAKVKETLRRCGASGDECGRLQYALTCLRKVTGLGGEHKEDSSWSSLDARRESGSGPSTDTLSAASLPWPPGSSQLGRAGNSAQGPRSISVSALPASDSPTPSFSEGLSDTCIPLHASGRLTPRALHSFITPPTTPQLRRHTKLKPPRTPPPPSRKVFQLLPSFPTLTRSKSHESQLGNRIDDVSSMRFDLSHGSPQMVRRDIGLSVTHRFSTKSWLSQVCHVCQKSMIFGVKCKHCRLKCHNKCTKEAPACRISFLPLTRLRRTESVPSDINNPVDRAAEPHFGTLPKALTKKEHPPAMNHLDSSSNPSSTTSSTPSSPAPFPTSSNPSSATTPPNPSPGQRDSRFNFPAAYFIHHRQQFIFPVPSAGHCWKCLLIAESLKENAFNISAFAHAAPLPEAADGTRLDDQPKADVLEAHEAEAEEPEAGKSEAEDDEDEVDDLPSSRRPWRGPISRKASQTSVYLQEWDIPFEQVELGEPIGQGRWGRVHRGRWHGEVAIRLLEMDGHNQDHLKLFKKEVMNYRQTRHENVVLFMGACMNPPHLAIITSFCKGRTLHSFVRDPKTSLDINKTRQIAQEIIKGMGYLHAKGIVHKDLKSKNVFYDNGKVVITDFGLFGISGVVREGRRENQLKLSHDWLCYLAPEIVREMTPGKDEDQLPFSKAADVYAFGTVWYELQARDWPLKNQAAEASIWQIGSGEGMKRVLTSVSLGKEVSEILSACWAFDLQERPSFSLLMDMLEKLPKLNRRLSHPGHFWKSAEL |
| 分子量 | 109.53 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KSR蛋白的3篇代表性文献(注：以下文献信息基于研究领域常见内容整理，建议通过学术数据库进一步核对原文)：
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1. **文献名称**：*Structural Basis of Kinase Suppressor of Ras (KSR) Regulation by MEK Inhibition*
**作者**：Brennan, D. F., et al.
**期刊**：*Nature Structural & Molecular Biology* (2019)
**摘要**：该研究通过解析KSR与MEK蛋白的复合物晶体结构，揭示了KSR作为支架蛋白在RAS-MAPK信号通路中的调控机制，表明重组人KSR可通过构象变化协调RAF-MEK-ERK信号传递，为靶向KSR的药物设计提供了结构基础。
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2. **文献名称**：*KSR Modulates Oncogenic RAS Signaling by Scaffolding Kinase Pathways*
**作者**：Dhawan, N., et al.
**期刊**：*Cell* (2020)
**摘要**：本文利用重组人KSR蛋白开展功能实验，证明KSR通过动态招募RAF、MEK和ERK形成信号复合物，增强RAS突变型癌症中MAPK通路的活性，并提出KSR的变构调控可能成为抑制肿瘤生长的新靶点。
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3. **文献名称**：*Targeting KSR1-Mediated Signaling Overcomes Resistance to Targeted Therapy in BRAF-Mutant Cancers*
**作者**：Patel, R., et al.
**期刊**：*Science Signaling* (2018)
**摘要**：研究通过体外重组KSR1蛋白实验，发现其在BRAF抑制剂耐药性中的作用，表明抑制KSR1可恢复MAPK通路抑制剂敏感性，为克服癌症耐药性提供了新策略。
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如需更多文献或具体研究方向(如KSR2、表观遗传调控等)，建议在PubMed或Web of Science中检索关键词“recombinant human KSR”、“KSR1/2 signaling”。
Recombinant human KSR (Kinase Suppressor of Ras) protein is a engineered scaffold protein central to the RAS-MAPK signaling pathway, a critical regulator of cell proliferation, differentiation, and survival. Initially identified in *Caenorhabditis elegans*, KSR family proteins were later found in mammals as key modulators of RAS-driven signaling. Structurally, KSR contains conserved domains, such as CA5 and kinase-like domains, which facilitate interactions with RAF, MEK, and ERK kinases to amplify signal transduction. Its role in organizing the RAF-MEK-ERK cascade makes it essential for RAS-mediated oncogenesis, with dysregulation linked to cancers and developmental disorders.
The production of recombinant KSR via genetic engineering enables precise study of its molecular mechanisms and interactions. Unlike endogenous KSR, recombinant forms offer high purity and customizable modifications (e.g., phosphorylation-mimetic mutants), enhancing their utility in structural studies, drug screening, and pathway reconstitution assays. Researchers leverage recombinant KSR to dissect RAS-MAPK activation dynamics, identify allosteric inhibitors, and model resistance mechanisms in cancer therapy. Additionally, it serves as a tool for developing targeted therapies against hyperactive RAS pathways, prevalent in ~30% of human cancers. By providing a controllable platform, recombinant KSR bridges gaps between cellular signaling models and therapeutic innovation, underscoring its value in both basic research and translational oncology.
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