| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KTI12 |
| Uniprot No | Q96EK9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354aa |
| 活性数据 | MPLVVFCGLPYSGKSRRAEELRVALAAEGRAVYVVDDAAVLGAEDPAVYGDSAREKALRGALRASVERRLSRHDVVILDSLNYIKGFRYELYCLARAARTPLCLVYCVRPGGPIAGPQVAGANENPGRNVSVSWRPRAEEDGRAQAAGSSVLRELHTADSVVNGSAQADVPKELEREESGAAESPALVTPDSEKSAKHGSGAFYSPELLEALTLRFEAPDSRNRWDRPLFTLVGLEEPLPLAGIRSALFENRAPPPHQSTQSQPLASGSFLHQLDQVTSQVLAGLMEAQKSAVPGDLLTLPGTTEHLRFTRPLTMAELSRLRRQFISYTKMHPNNENLPQLANMFLQYLSQSLH |
| 分子量 | 65 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KTI12蛋白的3篇示例参考文献(注：KTI12相关研究较少，以下内容为模拟示例，仅供参考)：
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1. **标题**：Expression and purification of recombinant human KTI12 in *E. coli* for functional analysis
**作者**：Li X, et al.
**摘要**：报道了通过大肠杆菌系统高效表达重组人KTI12蛋白的方法，优化了诱导条件与纯化步骤，获得高纯度蛋白并验证其体外RNA结合活性。
2. **标题**：Structural insights into KTI12's role in tRNA modification by X-ray crystallography
**作者**：Wang Y, et al.
**摘要**：通过重组KTI12蛋白的晶体结构解析，揭示其与tRNA修饰酶的相互作用位点，阐明其在翻译调控中的分子机制。
3. **标题**：KTI12 depletion impairs cell proliferation via disrupting ribosome biogenesis
**作者**：Garcia-Ruiz C, et al.
**摘要**：利用重组KTI12蛋白回补实验，证明KTI12通过调控核糖体RNA加工影响细胞周期进程，缺失导致HeLa细胞增殖停滞。
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注：实际研究中建议通过基因数据库(如UniProt ID搜索)或学术引擎确认KTI12的最新研究进展。
Recombinant human KTI12 protein is a genetically engineered form of the human KTI12 homolog, a conserved eukaryotic protein implicated in transcriptional regulation and post-translational modifications. The KTI12 gene, identified through homology with yeast counterparts (e.g., Saccharomyces cerevisiae Kti12), encodes a polypeptide that interacts with the Elongator complex, a multifunctional protein complex involved in tRNA modification, histone acetylation, and cellular stress responses. Human KTI12 is associated with modulating the activity of RNA polymerase II and regulating the elongation phase of transcription, impacting gene expression patterns critical for cell differentiation, development, and homeostasis.
Produced via heterologous expression systems (e.g., E. coli or mammalian cells), recombinant KTI12 enables functional studies to dissect its biochemical interactions, structural properties, and role in diseases. Emerging research links KTI12 dysregulation to neurological disorders and cancer progression, possibly via disrupted tRNA modification and protein synthesis pathways. Its recombinant form serves as a tool for in vitro assays, structural analysis (e.g., crystallography), and screening for therapeutic molecules targeting transcription-coupled pathologies. Further studies aim to clarify its regulatory networks and validate its potential as a biomarker or drug target.
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