| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KRTAP5-8 |
| Uniprot No | O75690 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-187aa |
| 活性数据 | MGCCGCSGGCGSGCGGCGSGCGGCGSSCCVPICCCKPVCCCVPACSCSSCGSCGGSKGGRGSCGGSKGDCGSCGGSKGGCGSCGCSQCSCYKPCCCSSGCGSSCCQSSCCKPCCSQSSCCKPCSCSSGCGSSCCQSSCCKPCCSQSSCCKPCCCSSGCGSSCCQSSCCKPCCSQSSCCVPICCQCKI |
| 分子量 | 20.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人KRTAP5-8蛋白的现有研究较为有限，以下是结合相关领域可能相关的文献方向或类似研究的示例(**注：以下为模拟示例，实际文献可能需要通过学术数据库检索确认**)：
1. **《Characterization of recombinant human KRTAP5-8 protein expression and its role in hair shaft formation》**
*作者：Smith J, et al.*
摘要：研究通过大肠杆菌系统成功表达重组人KRTAP5-8蛋白，发现其在体外模型中促进角蛋白交联，可能与毛发纤维的机械强度相关。
2. **《KRTAP5-8 genetic variations and recombinant protein functional analysis in alopecia》**
*作者：Li X, et al.*
摘要：分析了KRTAP5-8基因多态性与脱发疾病的关联，并通过重组蛋白实验表明其可能调控毛囊角质化过程。
3. **《Structural insights into human KRTAP5-8 protein using recombinant expression and NMR spectroscopy》**
*作者：Wang Y, et al.*
摘要：利用重组表达和核磁共振技术解析KRTAP5-8的结构，揭示其保守的α-螺旋域在角蛋白网络组装中的作用。
4. **《High-yield purification of recombinant KRTAP5-8 and its antioxidant activity in keratinocytes》**
*作者：Gomez R, et al.*
摘要：开发了重组KRTAP5-8的高效纯化方法，并发现其在氧化应激下保护角质细胞的潜在功能。
**建议**：若需真实文献，请使用关键词“recombinant KRTAP5-8”“human keratin-associated protein”在PubMed或Google Scholar中检索，或关注角蛋白家族(Keratin-associated proteins, KRTAPs)的功能研究。目前该蛋白可能尚属小众领域，建议拓展至KRTAP家族其他成员的相关研究。
The human keratin-associated protein 8 (KRTAP5-8) is a member of the keratin-associated protein (KAP) family, which plays a critical role in the structural organization and mechanical properties of hair fibers. These proteins are cysteine-rich and interact with keratin intermediate filaments in the hair cortex, contributing to the formation of disulfide bonds that stabilize the hair's rigidity and resilience. KRTAP5-8 is encoded within the type I high-sulfur KAP gene cluster on chromosome 11 and is expressed in the hair follicle during the differentiation of cortical cells. Its expression is tightly regulated and may vary depending on hair cycle stages or genetic polymorphisms.
Recombinant KRTAP5-8 is synthesized in vitro using genetic engineering techniques, often expressed in bacterial or mammalian systems for functional studies. Its recombinant form enables research into hair biology, particularly mechanisms underlying hair disorders like hypotrichosis or brittleness. Additionally, it serves as a model to investigate protein interactions within the hair shaft matrix and to develop biomaterials mimicking hair’s mechanical properties. Current studies also explore its potential role in signaling pathways affecting follicular development. However, its precise biochemical functions and post-translational modifications remain under investigation, highlighting the need for further structural and biophysical characterization.
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