| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHL23 |
| Uniprot No | Q8NBE8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-558aa |
| 活性数据 | MALKGQEDYIYLFKDSTHPVDFLDAFRTFYLDGLFTDITLQCPSGIIFHCHRAVLAACSNYFKAMFTADMKEKFKNKIKLSGIHHDILEGLVNYAYTSQIEITKRNVQSLLEAADLLQFLSVKKACERFLVRHLDIDNCIGMHSFAEFHVCPELEKESRRILCSKFKEVWQQEEFLEISLEKFLFILSRKNLSVWKEEAIIEPVIKWTAHDVENRIECLYNLLSYINIDIDPVYLKTALGLQRSCLLTENKIRSLIYNALNPMHKEISQRSTATMYIIGGYYWHPLSEVHIWDPLTNVWIQGAEIPDYTRESYGVTCLGPNIYVTGGYRTDNIEALDTVWIYNSESDEWTEGLPMLNARYYHCAVTLGGCVYALGGYRKGAPAEEAEFYDPLKEKWIPIANMIKGVGNATACVLHDVIYVIGGHCGYRGSCTYDKVQSYNSDINEWSLITSSPHPEYGLCSVPFENKLYLVGGQTTITECYDPEQNEWREIAPMMERRMECGAVIMNGCIYVTGGYSYSKGTYLQSIEKYDPDLNKWEIVGNLPSAMRSHGCVCVYNV |
| 分子量 | 90.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KLHL23蛋白的3篇示例参考文献(注：以下为虚构示例，实际文献需通过学术数据库查询)：
1. **文献名称**：*"Expression and Functional Characterization of Recombinant Human KLHL23 Protein in Ubiquitination Pathways"*
**作者**：Smith, J. et al.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人KLHL23蛋白，发现其通过与CUL3结合形成E3泛素连接酶复合体，调控特定底物蛋白的泛素化降解。
2. **文献名称**：*"Structural Analysis of KLHL23 Reveals Critical Domains for Substrate Recognition"*
**作者**：Li, X. & Zhang, Y.
**摘要**：通过X射线晶体学解析了重组人KLHL23蛋白的Kelch重复结构域，揭示其与底物结合的关键氨基酸残基，为疾病相关突变研究提供结构基础。
3. **文献名称**：*"KLHL23 Suppresses Tumor Growth via Modulation of the Wnt/β-Catenin Pathway"*
**作者**：Wang, H. et al.
**摘要**：研究发现重组人KLHL23蛋白在结肠癌细胞中过表达可促进β-catenin的泛素化降解，抑制Wnt信号通路活性，从而减缓肿瘤生长。
建议通过PubMed或Google Scholar检索最新文献，关键词："recombinant human KLHL23", "KLHL23 ubiquitin", "KLHL23 structure/function"。
Recombinant human KLHL23 protein is a genetically engineered form of the Kelch-like family member 23 (KLHL23), a substrate recognition component of the Cullin3-RING ubiquitin ligase (CRL3) complex. KLHL23 belongs to the KLHL protein family, characterized by conserved N-terminal BTB/POZ domains, BACK domains, and C-terminal Kelch repeat motifs involved in protein-protein interactions. This protein plays a regulatory role in ubiquitination-mediated proteasomal degradation, a critical process for maintaining cellular homeostasis, signaling, and stress responses. The recombinant variant is typically produced in Escherichia coli or mammalian expression systems, ensuring high purity and functionality for experimental applications. Studies suggest KLHL23 may interact with specific substrates to modulate biological pathways linked to cell cycle progression, DNA damage repair, or neuronal functions. Its dysregulation has been implicated in certain cancers and neurodegenerative disorders, making it a potential therapeutic target. Current research focuses on elucidating its structural motifs, substrate specificity, and physiological roles using recombinant KLHL23 in biochemical assays, protein interaction studies, and disease models. The availability of recombinant KLHL23 enables deeper insights into CRL3 complex mechanisms and contributes to drug discovery efforts targeting ubiquitin-proteasome pathways.
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