| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHL21 |
| Uniprot No | Q9UJP4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-597aa |
| 活性数据 | MERPAPLAVLPFSDPAHALSLLRGLSQLRAERKFLDVTLEAAGGRDFPAHRAVLAAASPYFRAMFAGQLRESRAERVRLHGVPPDMLQLLLDFSYTGRVAVSGDNAEPLLRAADLLQFPAVKEACGAFLQQQLDLANCLDMQDFAEAFSCSGLASAAQRFILRHVGELGAEQLERLPLARLLRYLRDDGLCVPKEEAAYQLALRWVRADPPRRAAHWPQLLEAVRLPFVRRFYLLAHVEAEPLVARCPPCLRLLREARDFQAARYDRHDRGPCPRMRPRPSTGLAEILVLVGGCDQDCDELVTVDCYNPQTGQWRYLAEFPDHLGGGYSIVALGNDIYVTGGSDGSRLYDCVWRYNSSVNEWAEVAPMLKAREYHSSSVPDGLLYVVAADSTERYDHTTDSWEALQPMTYPMDNCSTTACRGRLYAIGSLAGKETMVMQCYDPDTDLWSLVDCGQLPPWSFAPKTATLNGLMYFVRDDSAEVDVYNPTRNEWDKIPSMNQVHVGGSLAVLGGKLYVSGGYDNTFELSDVVEAYDPETRAWSVVGRLPEPTFWHGSVSIFRQFMPQTFSGGRGFELDSGSDDMDPGRPRPPRDPDELH |
| 分子量 | 93 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KLHL21蛋白的3篇参考文献的简要概括(基于已知研究方向整合，非真实文献)：
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1. **文献名称**：*KLHL21 interacts with Cullin3 to regulate cell cycle progression via ubiquitination of cyclin D1*
**作者**：Shimizu K, et al.
**摘要**：研究揭示了重组人KLHL21蛋白与Cullin3结合形成E3泛素连接酶复合体，通过靶向细胞周期蛋白Cyclin D1的泛素化降解调控G1/S期转换，为KLHL21在细胞周期中的作用提供机制解释。
2. **文献名称**：*Downregulation of KLHL21 in breast cancer correlates with poor prognosis and promotes tumor invasion*
**作者**：Liu Y, et al.
**摘要**：通过分析乳腺癌临床样本，发现KLHL21表达显著降低与患者生存率相关。体外实验表明重组人KLHL21过表达抑制肿瘤细胞迁移和侵袭，提示其抑癌蛋白功能。
3. **文献名称**：*Structural analysis of KLHL21 reveals a conserved BTB-Kelch domain essential for substrate recognition*
**作者**：Rotin D, et al.
**摘要**：利用X射线晶体学解析重组人KLHL21蛋白的三维结构，阐明其BTB和Kelch结构域在底物结合中的作用，并确定关键氨基酸残基对Cullin3复合体组装及泛素化活性的影响。
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*注：以上内容基于领域内常见研究方向模拟，实际文献需通过PubMed或Google Scholar检索确认。*
Recombinant human KLHL21 protein is a genetically engineered form of the Kelch-like protein 21 (KLHL21), a member of the Kelch-like (KLHL) family characterized by conserved BTB (Bric-a-brac, Tramtrack, Broad-complex) and BACK domains, along with C-terminal Kelch repeat motifs. These structural features enable KLHL21 to act as a substrate-specific adaptor for Cullin 3 (CUL3)-based E3 ubiquitin ligase complexes, facilitating the targeted ubiquitination and subsequent proteasomal degradation of proteins involved in diverse cellular processes. KLHL21 plays critical roles in regulating cell cycle progression, apoptosis, and cytoskeletal organization. Studies suggest its involvement in mitotic regulation, particularly through interactions with components of the chromosomal passenger complex (CPC), and its dysregulation has been linked to cancer progression and genomic instability.
The recombinant version is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells), often tagged with affinity markers like His or FLAG for purification and detection. This tool enables mechanistic studies of KLHL21’s substrate recognition, its role in ubiquitination pathways, and potential therapeutic targeting. Recent research highlights its tumor-suppressive functions in certain cancers, while paradoxically, its overexpression may promote metastasis in others, underscoring context-dependent roles. As a reagent, recombinant KLHL21 aids in elucidating pathways critical to cellular homeostasis and disease, offering avenues for drug discovery aimed at modulating ubiquitin-proteasome system activity.
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