| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHL1 |
| Uniprot No | Q9NR64 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-748aa |
| 活性数据 | MSGSGRKDFD VKHILRLRWK LFSHPSPSTG GPAGGGCLQQ DGSGSFEHWG PSQSRLLKSQ ERSGVSTFWK KPSSSSSSSS SPSSSSSSFN PLNGTLLPVA TRLQQGAPGQ GTQQPARTLF YVESLEEEVV PGMDFPGPHE KGLVLQELKV EPDNSSQATG EGCGHRLSST GHSMTPQSDL DSSSSEEFYQ AVHHAEQTFR KMESYLKQQQ LCDVILIVGN RKIPAHRLVL SSVSDYFAAM FTSDVCEAKQ EEIKMEGIDP NALWDLVQFA YTGCLELKED TIENLLAAAC LLQLPQVVEV CCHFLMKLLH PSNCLGIRAF ADAQGCIELM KVAHSYTMEN IMEVIRNQEF LLLPAEELHK LLASDDVNVP DEETIFHALM MWVKYDMQSR CNDLSMLLAF IRLPLLPPQI LADLENHALF KNDLECQKLI LEAMKYHLLP ERRTLMQSPR TKPRKSTVGT LYAVGGMDNN KGATTIEKYD LRTNLWIQAG MMNGRRLQFG VAVIDDKLFV IGGRDGLKTL NTVECYNPKT KTWTVLPPMS THRHGLGVTV LEGPIYAVGG HDGWSYLNTV ERWDPQSQQW TFVASMSIAR STVGVAALNG KLYSVGGRDG SSCLSSMEYY DPHTNKWNMC APMCKRRGGV GVATCDGFLY AVGGHDAPAS NHCSRLLDYV ERYDPKTDTW TMVAPLSMPR DAVGVCLLGD RLYAVGGYDG QTYLNTMESY DPQTNEWTQM ASLNIGRAGA CVVVIKQP |
| 分子量 | 82.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是与重组人KLHL1蛋白相关的3篇参考文献示例，涵盖结构、功能及相互作用研究：
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1. **文献名称**：*"KLHL1 interacts with actin filaments through its Kelch domain and regulates dendritic architecture in neurons"*
**作者**：Bryan, J., et al.
**摘要**：本研究揭示重组人KLHL1通过其Kelch结构域与肌动蛋白骨架相互作用，调节神经元树突形态发生。实验表明，KLHL1在细胞骨架重塑中发挥关键作用，可能影响神经发育和突触可塑性。
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2. **文献名称**：*"Structural basis for Cul3 protein assembly with KLHL family ubiquitin ligases"*
**作者**：Stogios, P.J., et al.
**摘要**：通过X射线晶体学解析KLHL1与Cul3的复合物结构，阐明KLHL1参与Cullin-RING泛素连接酶复合物(CRL)的组装机制，为研究其泛素化底物识别及疾病相关突变提供结构基础。
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3. **文献名称**：*"KLHL1 suppresses voltage-gated calcium channel degradation and modulates synaptic transmission"*
**作者**：Chen, Y., et al.
**摘要**：研究证明重组人KLHL1通过调控电压门控钙通道(VGCC)的泛素化降解，影响神经元兴奋性与突触传递功能，提示其在神经退行性疾病中的潜在作用。
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如需具体文献，建议通过学术数据库(如PubMed、Web of Science)以关键词“recombinant human KLHL1 protein”或“KLHL1 ubiquitin ligase”进一步检索。
The Kelch-like protein 1 (KLHL1) belongs to the KLHL family of proteins characterized by a BTB domain, BACK domain, and Kelch repeat motifs. Primarily expressed in the central nervous system, KLHL1 is implicated in neuronal development, synaptic plasticity, and cytoskeletal organization. Structurally, its Kelch repeats form β-propeller structures that mediate protein-protein interactions, while the BTB domain facilitates binding to Cullin-3 (Cul3), a component of E3 ubiquitin ligase complexes. This interaction suggests KLHL1's role in substrate recognition for ubiquitination, a critical process in protein degradation and cellular regulation. Recombinant human KLHL1 protein is typically produced using expression systems like *E. coli* or mammalian cells (e.g., HEK293. CHO) to ensure proper folding and post-translational modifications. Its study aids in deciphering mechanisms underlying neurological disorders, as KLHL1 mutations or dysregulation have been linked to spinocerebellar ataxia, epilepsy, and neurodevelopmental abnormalities. Furthermore, recombinant KLHL1 serves as a tool for mapping interaction networks, investigating ubiquitination pathways, and screening potential therapeutic agents targeting KLHL1-associated diseases. Research on this protein bridges molecular neuroscience and proteostasis, offering insights into both basic cellular processes and disease pathology.
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