| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHL22 |
| Uniprot No | Q53GT1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-634aa |
| 活性数据 | MAEEQEFTQLCKLPAQPSHPHCVNNTYRSAQHSQALLRGLLALRDSGILFDVVLVVEGRHIEAHRILLAASCDYFRGMFAGGLKEMEQEEVLIHGVSYNAMCQILHFIYTSELELSLSNVQETLVAACQLQIPEIIHFCCDFLMSWVDEENILDVYRLAELFDLSRLTEQLDTYILKNFVAFSRTDKYRQLPLEKVYSLLSSNRLEVSCETEVYEGALLYHYSLEQVQADQISLHEPPKLLETVRFPLMEAEVLQRLHDKLDPSPLRDTVASALMYHRNESLQPSLQSPQTELRSDFQCVVGFGGIHSTPSTVLSDQAKYLNPLLGEWKHFTASLAPRMSNQGIAVLNNFVYLIGGDNNVQGFRAESRCWRYDPRHNRWFQIQSLQQEHADLSVCVVGRYIYAVAGRDYHNDLNAVERYDPATNSWAYVAPLKREVYAHAGATLEGKMYITCGRRGEDYLKETHCYDPGSNTWHTLADGPVRRAWHGMATLLNKLYVIGGSNNDAGYRRDVHQVACYSCTSGQWSSVCPLPAGHGEPGIAVLDNRIYVLGGRSHNRGSRTGYVHIYDVEKDCWEEGPQLDNSISGLAACVLTLPRSLLLEPPRGTPDRSQADPDFASEVMSVSDWEEFDNSSED |
| 分子量 | 98.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KLHL22蛋白的3篇假设性参考文献示例(文献信息为模拟内容，仅供参考)：
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1. **文献名称**：**KLHL22 ubiquitinates Aurora A to regulate cell cycle progression in breast cancer cells**
**作者**：Zhang Y, Liu Q, Wang H
**摘要**：研究利用重组人KLHL22蛋白进行体外泛素化实验，发现其作为E3泛素连接酶复合物的底物识别亚基，靶向Aurora A激酶并促使其泛素化降解，从而抑制乳腺癌细胞的有丝分裂进程和增殖。
2. **文献名称**：**Structural basis for KLHL22-mediated recognition of substrate proteins**
**作者**：Tanaka K, Ito T, Nakagawa R
**摘要**：通过重组表达并解析KLHL22的Kelch结构域晶体结构，揭示其与靶蛋白相互作用的分子机制，为设计靶向KLHL22信号通路的抑制剂提供结构基础。
3. **文献名称**：**Development of a recombinant KLHL22 protein-based assay for drug discovery targeting E3 ligases**
**作者**：Chen L, Smith GP, Zhou M
**摘要**：研究报道了重组人KLHL22蛋白的高效表达及纯化方法，并建立基于该蛋白的高通量筛选平台，用于鉴定调控CRL3^(KLHL22)泛素连接酶活性的小分子化合物。
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### 备注：
以上文献为示例，实际研究需通过PubMed、Google Scholar等平台检索关键词(如“recombinant KLHL22”“KLHL22 ubiquitination”)。建议用户结合具体研究方向筛选近年文献，重点关注KLHL22在泛素化、癌症或细胞周期中的功能机制。
**Background of Recombinant Human KLHL22 Protein**
KLHL22 (Kelch-like protein 22) is a member of the KLHL family, characterized by a conserved BTB (Bric-a-brac, Tramtrack, Broad Complex) domain, a BACK (BTB and C-terminal Kelch) domain, and six Kelch-repeat motifs. These structural features enable KLHL22 to act as a substrate-specific adaptor in the Cullin-RING E3 ubiquitin ligase (CRL) complex, facilitating targeted protein ubiquitination and degradation via the ubiquitin-proteasome system.
KLHL22 has gained attention for its role in cellular processes such as cell cycle regulation, DNA damage response, and tumorigenesis. Studies suggest it interacts with proteins involved in mitotic progression, including Aurora kinase A, and may regulate chromosome alignment and spindle dynamics. Dysregulation of KLHL22 has been linked to cancer progression, particularly in breast and liver cancers, where it may influence tumor growth, metastasis, or chemoresistance.
Recombinant human KLHL22 protein is generated through genetic engineering (e.g., in *E. coli* or mammalian systems) to enable functional studies. Its applications include elucidating molecular mechanisms in ubiquitination pathways, screening for small-molecule modulators, and exploring therapeutic strategies targeting KLHL22-associated diseases. Recent research also highlights its potential role in immune regulation, particularly in modulating PD-L1 degradation, suggesting relevance in cancer immunotherapy.
Overall, recombinant KLHL22 serves as a critical tool for dissecting its physiological and pathological functions, with implications for drug discovery and precision medicine.
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