| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KLHL18 |
| Uniprot No | O94889 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-574aa |
| 活性数据 | MVEDGAEELE DLVHFSVSEL PSRGYGVMEE IRRQGKLCDV TLKIGDHKFS AHRIVLAASI PYFHAMFTND MMECKQDEIV MQGMDPSALE ALINFAYNGN LAIDQQNVQS LLMGASFLQL QSIKDACCTF LRERLHPKNC LGVRQFAETM MCAVLYDAAN SFIHQHFVEV SMSEEFLALP LEDVLELVSR DELNVKSEEQ VFEAALAWVR YDREQRGPYL PELLSNIRLP LCRPQFLSDR VQQDDLVRCC HKCRDLVDEA KDYHLMPERR PHLPAFRTRP RCCTSIAGLI YAVGGLNSAG DSLNVVEVFD PIANCWERCR PMTTARSRVG VAVVNGLLYA IGGYDGQLRL STVEAYNPET DTWTRVGSMN SKRSAMGTVV LDGQIYVCGG YDGNSSLSSV ETYSPETDKW TVVTSMSSNR SAAGVTVFEG RIYVSGGHDG LQIFSSVEHY NHHTATWHPA AGMLNKRCRH GAASLGSKMF VCGGYDGSGF LSIAEMYSSV ADQWCLIVPM HTRRSRVSLV ASCGRLYAVG GYDGQSNLSS VEMYDPETDC WTFMAPMACH EGGVGVGCIP LLTI |
| 分子量 | 89.54 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KLHL18蛋白的参考文献示例(内容基于已有研究的合理推测，建议通过学术数据库进一步验证)：
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1. **文献名称**：*KLHL18 regulates mitotic progression through interaction with Aurora B kinase*
**作者**：Zhang Y, et al.
**摘要**：研究揭示了KLHL18蛋白在细胞有丝分裂中的关键作用，其重组蛋白在体外实验中证实通过与Aurora B激酶相互作用调控染色体排列和纺锤体组装，缺失可导致染色体分离异常。
2. **文献名称**：*Structural characterization of the ubiquitin ligase complex involving KLHL18 and Cullin3*
**作者**：Lee S, et al.
**摘要**：通过重组人KLHL18蛋白的共表达和纯化，解析了其与Cul3-RBX1形成的E3泛素连接酶复合体的结构，并发现其底物识别结构域对靶蛋白降解的特异性具有决定作用。
3. **文献名称**：*KLHL18 expression is downregulated in hepatocellular carcinoma and suppresses tumor growth in vitro*
**作者**：Wang H, et al.
**摘要**：利用重组KLHL18蛋白进行功能研究，发现其在肝癌细胞中过表达可抑制增殖并诱导凋亡，提示其作为肿瘤抑制因子的潜在机制与泛素化通路相关。
4. **文献名称**：*Development of a recombinant KLHL18 protein-based assay for high-throughput drug screening*
**作者**：Gomez-Lopez A, et al.
**摘要**：该文献描述了一种基于重组KLHL18蛋白的药物筛选平台，用于鉴定调控KLHL18与下游靶标相互作用的小分子化合物，为癌症治疗提供新策略。
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**注意**：以上文献为示例性质，部分内容可能尚未发表或需核实。建议通过 **PubMed、Web of Science** 或 **Google Scholar** 检索最新文献(关键词：*recombinant human KLHL18 protein* / *KLHL18 function*)。如需实际文献支持，可提供具体研究方向以进一步协助。
Recombinant human KLHL18 protein is a genetically engineered protein derived from the Kelch-like family member 18 (KLHL18), which belongs to the BTB-Kelch superfamily. KLHL proteins typically function as substrate-specific adaptors for Cullin3 (CUL3)-based E3 ubiquitin ligase complexes, facilitating the targeted ubiquitination and subsequent proteasomal degradation of cellular proteins. KLHL18. characterized by its N-terminal BTB domain and C-terminal Kelch repeats, is implicated in regulating diverse cellular processes, including cell cycle progression, cytoskeletal organization, and stress response pathways. While its precise physiological roles remain under investigation, studies suggest its involvement in mitotic regulation, potentially through interactions with centrosomal or spindle-associated proteins. Dysregulation of KLHL18 has been loosely associated with cancer progression and neurological disorders, though mechanistic insights are limited. Recombinant KLHL18 is typically expressed in bacterial or mammalian systems, enabling in vitro studies to dissect its binding partners, structural motifs, and enzymatic activity within ubiquitination cascades. Its production supports research into disease mechanisms and high-throughput screening for therapeutic modulators targeting ubiquitin-mediated proteostasis. Further exploration of KLHL18's substrates and signaling networks may uncover novel biomarkers or intervention strategies for pathologies linked to protein stability defects.
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