| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIF2 |
| Uniprot No | O00139 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-679aa |
| 活性数据 | MVTSLNEDNESVTVEWIENGDTKGKEIDLESIFSLNPDLVPDEEIEPSPETPPPPASSAKVNKIVKNRRTVASIKNDPPSRDNRVVGSARARPSQFPEQSSSAQQNGSVSDISPVQAAKKEFGPPSRRKSNCVKEVEKLQEKREKRRLQQQELREKRAQDVDATNPNYEIMCMIRDFRGSLDYRPLTTADPIDEHRICVCVRKRPLNKKETQMKDLDVITIPSKDVVMVHEPKQKVDLTRYLENQTFRFDYAFDDSAPNEMVYRFTARPLVETIFERGMATCFAYGQTGSGKTHTMGGDFSGKNQDCSKGIYALAARDVFLMLKKPNYKKLELQVYATFFEIYSGKVFDLLNRKTKLRVLEDGKQQVQVVGLQEREVKCVEDVLKLIDIGNSCRTSGQTSANAHSSRSHAVFQIILRRKGKLHGKFSLIDLAGNERGADTSSADRQTRLEGAEINKSLLALKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMASCENTLNTLRYANRVKELTVDPTAAGDVRPIMHHPPNQIDDLETQWGVGSSPQRDDLKLLCEQNEEEVSPQLFTFHEAVSQMVEMEEQVVEDHRAVFQESIRWLEDEKALLEMTEEVDYDVDSYATQLEAILEQKIDILTELRDKVKSFRAALQEEEQASKQINPKRPRAL |
| 分子量 | 100.43 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIF2蛋白的3篇代表性文献及其摘要概括：
1. **"KIF2 Regulation of Mitotic Chromosome Alignment by Tubulin Depolymerization"**
- 作者：Ganem, N.J. & Compton, D.A.
- 摘要：研究揭示了KIF2(Kinesin-13家族成员)通过微管解聚活性调控有丝分裂中染色体排列的过程，重组人KIF2蛋白被用于体外微管动力学实验，表明其缺失会导致染色体排列缺陷。
2. **"Structure and Function of the Kinesin-13 Motor KIF2 in Neuronal Development"**
- 作者：Homma, N., Takei, Y., & Hirokawa, N.
- 摘要：利用重组人KIF2蛋白分析了其结构域功能，发现其通过微管解聚活性调控神经元轴突分支修剪，为神经发育障碍机制提供了新见解。
3. **"Recombinant KIF2A as a Therapeutic Target in Cancer Metastasis"**
- 作者：Li, X., et al.
- 摘要：研究通过重组人KIF2A蛋白验证其在肿瘤细胞迁移中的调控作用，抑制KIF2A可减少癌细胞侵袭，提示其作为抗癌治疗靶点的潜力。
(注：以上文献信息为示例，实际引用时请核对真实论文的准确性。)
**Background of Recombinant Human KIF2 Protein**
Kinesin family member 2 (KIF2) is a microtubule-depolymerizing motor protein belonging to the kinesin-13 family, which plays a pivotal role in regulating microtubule dynamics during cellular processes such as mitosis, neuronal development, and cell migration. Unlike conventional kinesins involved in cargo transport, KIF2 utilizes ATP hydrolysis to destabilize microtubule ends, facilitating structural remodeling critical for chromosome segregation, spindle positioning, and axon guidance.
Recombinant human KIF2 protein, produced via genetic engineering in systems like *E. coli* or mammalian cells, enables detailed study of its molecular mechanisms and interactions. Its recombinant form retains functional domains, including the conserved kinesin motor domain, ensuring activity in *in vitro* assays. Research highlights its involvement in diseases: dysregulated KIF2 expression correlates with cancers, neurodevelopmental disorders, and ciliopathies. For example, KIF2A deletion disrupts cortical neuron migration, while KIF2B overexpression is linked to tumor progression.
Recent advances in structural biology using recombinant KIF2 have clarified its ATPase cycle and microtubule-binding interfaces, aiding drug discovery. Additionally, it serves as a tool for screening therapeutic inhibitors targeting mitotic errors or pathological microtubule dynamics. The development of recombinant KIF2 underscores its biomedical relevance, bridging mechanistic insights into cellular dynamics and potential clinical applications.
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