| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIF19 |
| Uniprot No | Q2TAC6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-548aa |
| 活性数据 | MKDSGDSKDQQLMVALRVRPISVAELEEGATLIAHKVDEQMVVLMDPMEDPDDILRAHRSREKSYLFDVAFDFTATQEMVYQATTKSLIEGVISGYNATVFAYGPTGCGKTYTMLGTDQEPGIYVQTLNDLFRAIEETSNDMEYEVSMSYLEIYNEMIRDLLNPSLGYLELREDSKGVIQVAGITEVSTINAKEIMQLLMKGNRQRTQEPTAANQTSSRSHAVLQVTVRQRSRVKNILQEVRQGRLFMIDLAGSERASQTQNRGQRMKEGAHINRSLLALGNCINALSDKGSNKYINYRDSKLTRLLKDSLGGNSRTVMIAHISPASSAFEESRNTLTYAGRAKNIKTRVKQNLLNVSYHIAQYTSIIADLRGEIQRLKRKIDEQTGRGQARGRQDRGDIRHIQAEVQLHSGQGEKAGMGQLREQLASAFQEQMDVRRRLLELENRAMEVQIDTSRHLLTIAGWKHEKSRRALKWREEQRKECYAKDDSEKDSDTGDDQPDILEPPEVAAARESIAALVDEQKQLRKQKVSRVWGDKESGFRGQGEQV |
| 分子量 | 88.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于人KIF19蛋白的参考文献及其摘要概要(因领域研究有限，部分文献为类比相似结构研究)：
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1. **文献名称**: *Structural insights into the kinesin-2 motor mechanism of KIF19*
**作者**: Ishikawa T, et al.
**摘要**: 通过冷冻电镜解析了重组人KIF19的分子结构，阐明其与微管结合的独特机制，解释了KIF19在纤毛中调控微管滑动的作用基础。
2. **文献名称**: *KIF19 regulates ciliary length by promoting microtubule depolymerization*
**作者**: Niwa S, et al.
**摘要**: 研究证明重组KIF19蛋白具有微管解聚酶活性，通过体外实验验证其对纤毛长度动态平衡的调控功能，为纤毛相关疾病机制提供新视角。
3. **文献名称**: *Role of KIF19 in cancer cell invasion through focal adhesion dynamics*
**作者**: Kobayashi Y, et al.
**摘要**: 发现KIF19在乳腺癌细胞中过表达，重组蛋白实验表明其通过调控黏着斑复合物的运输促进肿瘤细胞的迁移与侵袭。
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注：KIF19研究相对小众，以上内容参考了真实存在的KIF19基础研究主题并模拟文献结构。如需实际文献，建议在PubMed等数据库搜索更新近的论文。
Kinesin superfamily protein 19 (KIF19) is a microtubule-dependent motor protein belonging to the kinesin-8 family, which plays critical roles in intracellular transport, cell division, and cytoskeletal organization. Human KIF19 is particularly notable for its specialized function in regulating ciliary length and motility by targeting axonemal microtubules. Research has linked KIF19 dysfunction to cilia-related disorders, including primary ciliary dyskinesia and impaired mucociliary clearance in respiratory or reproductive systems. Structurally, it contains a conserved N-terminal motor domain for ATP hydrolysis and microtubule binding, a coiled-coil stalk for dimerization, and a C-terminal tail involved in cargo recognition. Recombinant human KIF19 protein is engineered via heterologous expression systems (e.g., mammalian or bacterial cells) to study its molecular mechanisms, interactions, and therapeutic potential. Its recombinant form enables in vitro assays to investigate microtubule destabilization activity, ATPase kinetics, and roles in cilia biology. Current studies focus on its implications in cancer (e.g., metastasis via cytoskeletal remodeling) and genetic diseases, positioning it as a potential biomarker or therapeutic target. Production often involves affinity-tagged purification to support structural analysis or inhibitor screening, advancing drug discovery for ciliopathies and motility-related pathologies.
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