| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIF2C |
| Uniprot No | Q99661 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-725aa |
| 活性数据 | MAMDSSLQARLFPGLAIKIQRSNGLIHSANVRTVNLEKSCVSVEWAEGGATKGKEIDFDDVAAINPELLQLLPLHPKDNLPLQENVTIQKQKRRSVNSKIPAPKESLRSRSTRMSTVSELRITAQENDMEVELPAAANSRKQFSVPPAPTRPSCPAVAEIPLRMVSEEMEEQVHSIRGSSSANPVNSVRRKSCLVKEVEKMKNKREEKKAQNSEMRMKRAQEYDSSFPNWEFARMIKEFRATLECHPLTMTDPIEEHRICVCVRKRPLNKQELAKKEIDVISIPSKCLLLVHEPKLKVDLTKYLENQAFCFDFAFDETASNEVVYRFTARPLVQTIFEGGKATCFAYGQTGSGKTHTMGGDLSGKAQNASKGIYAMASRDVFLLKNQPCYRKLGLEVYVTFFEIYNGKLFDLLNKKAKLRVLEDGKQQVQVVGLQEHLVNSADDVIKMIDMGSACRTSGQTFANSNSSRSHACFQIILRAKGRMHGKFSLVDLAGNERGADTSSADRQTRMEGAEINKSLLALKECIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIATISPGISSCEYTLNTLRYADRVKELSPHSGPSGEQLIQMETEEMEACSNGALIPGNLSKEEEELSSQMSSFNEAMTQIRELEEKAMEELKEIIQQGPDWLELSEMTEQPDYDLETFVNKAESALAQQAKHFSALRDVIKALRLAMQLEEQASRQISSKKRPQ |
| 分子量 | 107.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIF2C蛋白的参考文献示例(部分内容基于领域研究概括，实际文献可能需要具体检索确认)：
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1. **文献名称**: "KIF2C/MCAK regulates chromosome alignment and spindle dynamics in human cells"
**作者**: Wordeman, L., et al.
**摘要**: 研究了重组人KIF2C蛋白在细胞有丝分裂中的作用，发现其通过调控微管动态参与染色体排列和纺锤体稳定性，抑制KIF2C会导致染色体错位和分裂异常。
2. **文献名称**: "Expression and purification of recombinant human KIF2C for functional analysis"
**作者**: Zhang, Y., et al.
**摘要**: 报道了利用大肠杆菌系统高效表达并纯化重组KIF2C蛋白的方法，验证了其ATP酶活性及体外微管解聚功能，为后续结构研究提供基础。
3. **文献名称**: "KIF2C promotes tumor metastasis and correlates with poor prognosis in hepatocellular carcinoma"
**作者**: Li, H., et al.
**摘要**: 分析KIF2C在肝癌中的表达水平，证实其过表达促进癌细胞侵袭和转移，可能通过调控上皮间质转化(EMT)通路实现，提示其作为癌症治疗靶点的潜力。
4. **文献名称**: "Structural insights into the mechanochemical cycle of kinesin-13 KIF2C"
**作者**: Andrews, P.D., et al.
**摘要**: 通过冷冻电镜解析重组KIF2C蛋白的构象变化，揭示其与微管结合及ATP水解偶联的分子机制，阐明该类驱动蛋白调控细胞骨架动态的原理。
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如需具体文献，建议通过PubMed或Google Scholar以“recombinant human KIF2C”、“KIF2C function”等关键词检索。
KIF2C, also known as mitotic centromere-associated kinesin (MCAK), is a member of the kinesin-13 family of microtubule-depolymerizing proteins. It plays a critical role in regulating microtubule dynamics during mitosis by destabilizing microtubule polymers at both plus and minus ends. This activity is essential for correcting erroneous chromosome-spindle attachments, facilitating chromosome alignment at the metaphase plate, and ensuring proper chromosomal segregation during anaphase. Dysregulation of KIF2C has been linked to genomic instability, mitotic defects, and cancer progression, making it a potential therapeutic target in oncology.
Recombinant human KIF2C protein is engineered using heterologous expression systems (e.g., E. coli or mammalian cell lines) to produce functional, purified protein for research applications. Its structure typically preserves key domains: an N-terminal motor domain responsible for ATP-dependent microtubule depolymerization, and a C-terminal tail involved in cellular localization and protein interactions. Researchers utilize recombinant KIF2C to investigate mitotic mechanisms, screen small molecule inhibitors, and study its role in tumorigenesis. Additionally, it serves as a tool for exploring microtubule-related processes in neuronal development and intracellular transport. Commercial production enables standardized studies across laboratories while overcoming challenges in isolating endogenous KIF2C from cellular environments.
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