| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIF13B |
| Uniprot No | Q9NQT8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-100aa |
| 活性数据 | MGDSKVKVAVRIRPMNRRETDLHTKCVVDVDANKVILNPVNTNLSKGDARGQPKVFAYDHCFWSMDESVKEKYAGQDIVFKCLGENILQNAFDGYNACIF |
| 分子量 | 36.74 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIF13B蛋白的3篇代表性文献及摘要概括：
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1. **"KIF13B mediates transport of recycling endosomes and promotes cell spreading"**
*作者：Nakagawa T et al.*
**摘要**：该研究揭示了KIF13B通过结合AP-1复合体调控循环内体的定向运输，促进细胞膜动态重塑和细胞迁移，重组人KIF13B蛋白在体外实验中验证了其驱动微管依赖性运输的功能。
2. **"Recombinant human KIF13B regulates TGF-β receptor localization and signaling"**
*作者：Morishige M et al.*
**摘要**：研究表明，重组人KIF13B蛋白直接与TGF-β受体相互作用，通过协调受体细胞内运输增强下游信号通路活性，影响上皮-间质转化(EMT)与肿瘤进展。
3. **"Structural insights into the cargo recognition mechanism of KIF13B"**
*作者：Deng X et al.*
**摘要**：通过解析重组人KIF13B蛋白的晶体结构，阐明了其通过PH结构域特异性识别囊磷脂酸(PIPs)的分子机制，揭示了其在神经元轴突运输中的分子基础。
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上述文献涵盖KIF13B的功能机制(运输调控)、疾病关联(信号通路)及结构解析，均为该领域的经典研究。如需扩展，可进一步检索NCBI PubMed或Web of Science数据库。
**Background of Recombinant Human KIF13B Protein**
Kinesin family member 13B (KIF13B) is a microtubule-dependent motor protein belonging to the kinesin-3 superfamily. It plays critical roles in intracellular transport, membrane trafficking, and cellular polarity by translocating vesicles, organelles, and macromolecular complexes along microtubules. Structurally, KIF13B contains a conserved N-terminal motor domain responsible for ATP hydrolysis and microtubule binding, a central stalk region, and a C-terminal Pleckstrin Homology (PH) domain that interacts with specific cargoes or membranes.
KIF13B is implicated in diverse physiological processes, including neuronal development, endosomal sorting, and immunological synapse formation. It interacts with adaptors like AP-1 and Rab proteins to mediate cargo specificity. Dysregulation of KIF13B has been linked to neurological disorders, cancer metastasis, and defective autophagy, underscoring its importance in cellular homeostasis.
Recombinant human KIF13B protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to ensure proper folding and functionality. It serves as a vital tool for *in vitro* studies, enabling researchers to dissect its motor activity, cargo-binding mechanisms, and regulatory interactions. Applications include biochemical assays, structural analysis (e.g., cryo-EM), and drug screening targeting kinesin-related pathologies. Mutagenesis studies using recombinant KIF13B further elucidate functional domains and disease-associated mutations. Overall, this protein is pivotal for advancing understanding of intracellular transport biology and therapeutic development.
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