| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIF2B |
| Uniprot No | Q8N4N8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-673aa |
| 活性数据 | MASQFCLPESPCLSPLKPLKPHFGDIQEGIYVAIQRSDKRIHLAVVTEINRENYWVTVEWVEKAVKKGKKIDLETILLLNPALDSAEHPMPPPPLSPLALAPSSAIRDQRTATKWVAMIPQKNQTASGDSLDVRVPSKPCLMKQKKSPCLWEIQKLQEQREKRRRLQQEIRARRALDVNTRNPNYEIMHMIEEYRRHLDSSKISVLEPPQEHRICVCVRKRPLNQRETTLKDLDIITVPSDNVVMVHESKQKVDLTRYLQNQTFCFDHAFDDKASNELVYQFTAQPLVESIFRKGMATCFAYGQTGSGKTYTMGGDFSGTAQDCSKGIYALVAQDVFLLLRNSTYEKLDLKVYGTFFEIYGGKVYDLLNWKKKLQVLEDGNQQIQVVGLQEKEVCCVEEVLNLVEIGNSCRTSRQTSVNAHSSRSHAVFQIILKSGGIMHGKFSLVDLAGNERGADTTKASRKRQLEGAEINKSLLALKECILALGQNKPHTPFRASKLTLVLRDSFIGQNSSTCMIATISPGMTSCENTLNTLRYANRVKKLNVDVRPYHRGHYPIGHEAPRMLKSHIGNSEMSLQRDEFIKIPYVQSEEQKEIEEVETLPTLLGKDTTISGKGSSQWLENIQERAGGVHHDIDFCIARSLSILEQKIDALTEIQKKLKLLLADLHVKSKVE |
| 分子量 | 74.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于重组人KIF2B蛋白的模拟参考文献示例(注：实际文献需通过学术数据库查询)：
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1. **文献名称**：*KIF2B regulates mitotic chromosome alignment by depolymerizing microtubules*
**作者**：Hirokawa, N. et al.
**摘要**：研究发现重组人KIF2B蛋白通过微管解聚活性调控有丝分裂中染色体的排列，揭示其在细胞分裂过程中维持基因组稳定的关键作用。
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2. **文献名称**：*Role of recombinant KIF2B in cancer cell migration and metastasis*
**作者**：Zhang, Y. et al.
**摘要**：通过体外实验证实重组KIF2B蛋白通过调控微管动态促进肿瘤细胞迁移，表明其可能在癌症转移中作为潜在治疗靶点。
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3. **文献名称**：*Structural analysis of recombinant human KIF2B reveals ATPase-dependent microtubule destabilization*
**作者**：Saito, K. & Tanaka, T.
**摘要**：通过冷冻电镜解析重组KIF2B蛋白结构，阐明了其依赖ATP水解的微管解聚机制，为开发相关抑制剂提供结构基础。
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**建议**：实际文献可通过PubMed、Google Scholar等平台搜索关键词“recombinant KIF2B”、“KIF2B microtubule”或“KIF2B cancer”获取最新研究。
Recombinant human KIF2B is a genetically engineered protein derived from the kinesin superfamily member 2B (KIF2B), a microtubule-associated motor protein involved in mitotic cell division. As a member of the kinesin-13 family, KIF2B plays a critical role in regulating microtubule dynamics by depolymerizing spindle microtubules, ensuring proper chromosomal alignment and segregation during mitosis. It is essential for maintaining genomic stability and preventing aneuploidy, a hallmark of many cancers.
Research has linked KIF2B dysregulation to tumorigenesis, particularly in cancers with chromosomal instability. Overexpression or mutations in KIF2B may disrupt mitotic fidelity, promoting uncontrolled cell proliferation and metastasis. This protein is thus studied both as a potential oncogenic driver and a therapeutic target. Recombinant KIF2B, typically produced in bacterial or mammalian expression systems, retains the functional domains required for microtubule interaction and ATPase activity. It is widely utilized in biochemical assays, structural studies, and drug discovery to investigate its mechanistic roles in mitosis and cancer progression. Additionally, it serves as a tool for exploring interactions with regulatory proteins or small-molecule inhibitors. Its recombinant form enables standardized research into KIF2B-related pathways, advancing our understanding of cell cycle regulation and anticancer strategies.
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