| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIF11 |
| Uniprot No | P52732 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 947-1056aa |
| 活性数据 | LLDQLKRKQPELLMMLNCSENNKEETIPDVDVEEAVLGQYTEEPLSQEPSVDAGVDCSSIGGVPFFQHKKSHGKDKENRGINTLERSKVEETTEHLVTKSRLPLRAQINL |
| 分子量 | 37.84 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIF11蛋白的3-4条参考文献示例(内容为模拟，非真实文献)：
1. **文献名称**：*Structural Insights into Human KIF11 Motor Domain and Its Interaction with Microtubules*
**作者**：Smith A, et al.
**摘要**：通过冷冻电镜解析重组人KIF11蛋白的ATP结合结构域三维结构，揭示其与微管结合的关键位点及ATP水解驱动运动的分子机制。
2. **文献名称**：*KIF11 Inhibition as a Therapeutic Strategy in Cancer Cell Proliferation*
**作者**：Johnson B, et al.
**摘要**：研究发现，重组人KIF11蛋白在癌细胞有丝分裂中起核心作用，靶向抑制KIF11可诱导癌细胞周期停滞并引发凋亡。
3. **文献名称**：*Development of a High-Yield Expression System for Recombinant Human KIF11 in E. coli*
**作者**：Chen L, et al.
**摘要**：优化重组人KIF11蛋白的大肠杆菌表达体系，获得高纯度蛋白，为体外筛选抗有丝分裂药物提供稳定材料。
4. **文献名称**：*KIF11 Mutations in Neurodevelopmental Disorders: Functional Validation Using Recombinant Protein Models*
**作者**：Wang Y, et al.
**摘要**：利用重组KIF11蛋白模拟患者突变体，证实特定突变导致微管结合能力缺陷，与脑发育异常相关。
(注：以上文献为示例性概括，实际文献需通过PubMed/Google Scholar等平台检索。)
**Background of Recombinant Human KIF11 Protein**
KIF11. also known as kinesin family member 11 or Eg5. is a motor protein belonging to the kinesin superfamily. It plays a critical role in mitosis by regulating spindle formation and ensuring proper chromosome segregation during cell division. Structurally, KIF11 contains a conserved N-terminal motor domain that hydrolyzes ATP to generate mechanical force, a central coiled-coil stalk for dimerization, and a C-terminal tail involved in cargo binding.
Dysregulation of KIF11 is linked to genomic instability and mitotic errors, contributing to cancer progression. Its overexpression in tumors and essential role in proliferation make it a promising therapeutic target. Small-molecule inhibitors (e.g., ispinesib) targeting KIF11 have been explored in clinical trials for anticancer therapies.
Recombinant human KIF11 protein is engineered using heterologous expression systems (e.g., *E. coli* or insect cells) to produce high-purity, functional protein for biochemical and cellular studies. It enables researchers to investigate KIF11’s enzymatic activity, interaction partners, and inhibitor screening. Additionally, recombinant KIF11 supports structural studies (e.g., crystallography) to elucidate mechanisms of drug resistance and guide rational drug design.
This protein is vital for advancing cancer research, understanding mitotic regulation, and developing targeted therapies against KIF11-dependent malignancies.
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