| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIF12 |
| Uniprot No | Q96FN5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-646aa |
| 活性数据 | MEERGSPDGD LARSLEQGPE GPETPIQVVL RVRPMSAAEL RRGQQSVLHC SGTRTLQGGP EVAFRFGAVL DAARTQEDVF RACGVRRLGE LALRGFSCTV FTFGQTGSGK TYTLTGPPPQ GEGVPVPPSL AGIMQRTFAW LLDRVQHLGA PVTLRASYLE IYNEQVRDLL SLGSPRPLPV RWNKTRGFYV EQLRVVEFGS LEALMELLQT GLSRRRNSAH TLNQASSRSH ALLTLYISRQ TAQQMPSVDP GEPPVGGKLC FVDLAGSEKV AATGSRGELM LEANSINRSL LALGHCISLL LDPQRKQSHI PFRDSKLTKL LADSLGGRGV TLMVACVSPS AQCLPETLST LRYASRAQRV TTRPQAPKSP VAKQPQRLET EMLQLQEENR RLQFQLDQMD CKASGLSGAR VAWAQRNLYG MLQEFMLENE RLRKEKSQLQ NSRDLAQNEQ RILAQQVHAL ERRLLSACYH HQQGPGLTPP CPCLMAPAPP CHALPPLYSC PCCHICPLCR VPLAHWACLP GEHHLPQVLD PEASGGRPPS ARPPPWAPPC SPGSAKCPRE RSHSDWTQTR VLAEMLTEEE VVPSAPPLPV RPPKTSPGLR GGAGVPNLAQ RLEALRDQIG SSLRRGRSQP PCSEGARSPG QVLPPH |
| 分子量 | 70.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIF12蛋白的3篇模拟参考文献(基于领域内典型研究主题，部分信息可能为假设性概括)：
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1. **文献名称**: *Expression and functional characterization of recombinant human KIF12 in microtubule dynamics*
**作者**: Suzuki M, et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化重组人KIF12蛋白，验证其与微管结合的能力。通过体外实验发现，KIF12通过ATP依赖的微管滑动活性调控细胞有丝分裂过程中的纺锤体组装，暗示其在细胞周期调控中的关键作用。
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2. **文献名称**: *KIF12 mutations cause cholestasis by impairing hepatic polarization*
**作者**: Li Y, et al.
**摘要**: 发现KIF12基因突变与婴儿肝内胆汁淤积症相关。通过构建重组人KIF12突变体，证实其与微管锚定蛋白ACBD5的互作缺陷，导致肝细胞极性丧失和胆汁分泌障碍，为疾病机制提供了分子解释。
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3. **文献名称**: *Proteomic analysis identifies KIF12 as a novel regulator of ciliogenesis*
**作者**: Guo J, et al.
**摘要**: 利用重组KIF12蛋白进行互作组学分析，发现其与纤毛基体蛋白CEP290结合。功能实验表明，KIF12通过协调纤毛母体复合物的微管运输，调控纤毛形成缺陷相关疾病的病理过程。
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**备注**：以上文献内容为领域内常见研究方向的模拟概括，实际文献需通过数据库(PubMed、Web of Science)检索确认。如需真实文献，请提供更具体的研究方向(如疾病、结构或互作蛋白)，以便进一步筛选。
Recombinant human KIF12 protein is a engineered form of the kinesin superfamily member 12 (KIF12), a microtubule-dependent motor protein involved in intracellular transport and organelle positioning. KIF12 belongs to the kinesin-3 subfamily, characterized by a conserved N-terminal motor domain that binds ATP and microtubules, enabling directional movement along microtubule tracks. The native protein contains coiled-coil domains for dimerization and cargo-binding regions that mediate interactions with specific vesicles or organelles. Studies suggest its role in neuronal development and hepatic function, with mutations linked to cholestatic disorders like progressive familial intrahepatic cholestasis (PFIC).
The recombinant version is typically produced in bacterial or mammalian expression systems, tagged with affinity markers (e.g., His, GST) for purification. It serves as a tool to study KIF12’s biochemical properties, interaction partners, and regulatory mechanisms in vitro. Research highlights its involvement in microtubule dynamics, vesicle trafficking, and cellular polarity establishment. Recent interest focuses on its pathological relevance—KIF12 mutations disrupt bile canaliculi formation in hepatocytes, implicating it in liver disease pathogenesis. Recombinant KIF12 enables drug screening and structural analysis (e.g., cryo-EM) to explore therapeutic targeting. However, full functional characterization remains ongoing due to overlapping roles with other kinesins and tissue-specific expression patterns.
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