| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0226 |
| Uniprot No | Q9H714 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-662aa |
| 活性数据 | MVSQSTVRQD SPVEPWEGIS DHSGIIDGSP RLLNTDHPPC QLDIRLMRHK AVWINPQDVQ QQPQDLQSQV PAAGNSGTHF VTDAASPSGP SPSCLGDSLA ETTLSEDTTD SVGSASPHGS SEKSSSFSLS STEVHMVRPG YSHRVSLPTS PGILATSPYP ETDSAFFEPS HLTSAADEGA VQVSRRTISS NSFSPEVFVL PVDVEKENAH FYVADMIISA MEKMKCNILS QQQTESWSKE VSGLLGSDQP DSEMTFDTNI KQESGSSTSS YSGYEGCAVL QVSPVTETRT YHDVKEICKC DVDEFVILEL GDFNDITETC SCSCSSSKSV TYEPDFNSAE LLAKELYRVF QKCWILSVVN SQLAGSLSAA GSIVVNEECV RKDFESSMNV VQEIKFKSRI RGTEDWAPPR FQIIFNIHPP LKRDLVVAAQ NFFCAGCGTP VEPKFVKRLR YCEYLGKYFC DCCHSYAESC IPARILMMWD FKKYYVSNFS KQLLDSIWHQ PIFNLLSIGQ SLYAKAKELD RVKEIQEQLF HIKKLLKTCR FANSALKEFE QVPGHLTDEL HLFSLEDLVR IKKGLLAPLL KDILKASLAH VAGCELCQGK GFICEFCQNT TVIFPFQTAT CRRCSACRAC FHKQCFQSSE CPRCARITAR RKLLESVASA AT |
| 分子量 | 73.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0226蛋白(RUBICON)的3篇代表性文献，涵盖其功能、相互作用及调控机制的研究：
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1. **文献名称**：*RUBICON is a critical regulator of autophagosome maturation*
**作者**：Matsunaga, K., et al.
**摘要**：
本研究揭示了RUBICON(KIAA0226)通过抑制Rab7 GTPase活性调控自噬体与溶酶体融合的关键作用。研究者利用重组人RUBICON蛋白进行体外结合实验，证明其直接与Rab7相互作用，并延缓自噬通量的完成，为自噬晚期阶段的分子机制提供了新见解。
2. **文献名称**：*Regulation of autophagy by RUBICON through interaction with Beclin 1*
**作者**：Zhong, Y., et al.
**摘要**：
该研究通过重组RUBICON蛋白的共表达实验，发现其与Beclin-1复合物的结合抑制III类PI3K激酶活性，进而负调控自噬起始。此结果解释了RUBICON在代谢应激条件下平衡细胞存活与死亡的分子基础。
3. **文献名称**：*Structural basis of RUBICON-mediated membrane curvature sensing in autophagy*
**作者**：Lee, Y.K., et al.
**摘要**：
研究者利用重组RUBICON蛋白进行结构解析(冷冻电镜技术)，揭示了其N端结构域通过识别膜曲率参与自噬体膜重塑的机制，为靶向RUBICON的疾病治疗策略提供了结构生物学依据。
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**说明**：
上述文献为基于RUBICON蛋白真实研究方向(自噬调控)的概括性示例，实际引用时建议通过PubMed或Google Scholar以“RUBICON autophagy”或“KIAA0226 recombinant”等关键词检索具体论文，并核对作者及发表年份。早期关键研究多聚焦于功能解析，近期研究偏向结构机制及疾病关联。
Recombinant human KIAA0226. also known as Rubicon (Run domain Beclin-1-interacting and cysteine-rich containing protein), is a protein implicated in cellular autophagy and endosomal trafficking. Initially identified through genomic sequencing, its biological role was later linked to the regulation of autophagy, a critical process for cellular homeostasis. Rubicon interacts with the Beclin-1-PI3KC3 complex, acting as a negative regulator of autophagosome maturation by inhibiting VPS34 lipid kinase activity. This interaction places it within key pathways like LC3-associated phagocytosis (LAP) and non-canonical autophagy, which are involved in pathogen clearance, inflammation, and organelle turnover.
Structurally, KIAA0226 contains a RUN domain (found in proteins involved in Ras-like signaling) and a cysteine-rich region, both critical for its molecular interactions. Dysregulation of KIAA0226 has been associated with diseases such as cancer, neurodegenerative disorders, and immune dysfunctions. Recombinant forms of KIAA0226 are typically produced in engineered cell systems (e.g., HEK293 or E. coli) for functional studies, allowing researchers to probe its mechanistic roles or screen therapeutic modulators. Its dual involvement in autophagy suppression and endolysosomal trafficking makes it a target for understanding pathologies linked to impaired protein degradation or membrane dynamics. Ongoing research explores its therapeutic potential in conditions involving aberrant autophagy, including infectious diseases and metabolic syndromes.
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