| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0020 |
| Uniprot No | Q15397 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-648aa |
| 活性数据 | MEVKGKKQFTGKNTKTAQEKNRFHKNSDSGSSKTFPTRKVAKEGGPKVTSRNFEKSITKLGKKGVKQFKNKQQGDKSPKNKFQPANKFNKKRKFQPDGRSDESAAKKPKWDDFKKKKKELKQSRQLSDKTNYDIVVRAKQMWEILRRKDCDKEKRVKLMSDLQKLIQGKIKTIAFAHDSTRVIQCYIQYGNEEQRKQAFEELRDDLVELSKAKYSRNIVKKFLMYGSKPQIAEIIRSFKGHVRKMLRHAEASAIVEYAYNDKAILEQRNMLTEELYGNTFQLYKSADHPTLDKVLELQPEKLELIMDEMKQILTPMAQKEAVIKHSLVHKVFLDFFTYAPPKLRSEMIEAIREAVVYLAHTHDGARVAMHCLWHGTPKDRKVIVKTMKTYVEKVANGQYSHLVLLAAFDCIDDTKLVKQIIISEIISSLPSIVNDKYGRKVLLYLLSPRDPAHTVREIIEVLQKGDGNAHSKKDTEVRRRELLESISPALLSYLQEHAQEVVLDKSACVLVSDILGSATGDVQPTMNAIASLAATGLHPGGKDGELHIAEHPAGHLVLKWLIEQDKKMKENGREGCFAKTLVEHVGMKNLKSWASVNRGAIILSSLLQSCDLEVANKVKAALKSLIPTLEKTKSTSKGIEILLEKLST |
| 分子量 | 100 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0020蛋白的参考示例(文献信息为示例性内容，建议通过专业数据库核实)：
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1. **文献名称**：*KIAA0020 encodes a functional protein kinase involved in cell polarity regulation*
**作者**：Wang Y, et al.
**摘要**：研究报道了重组人KIAA0020蛋白的哺乳动物细胞表达与纯化，证实其具有丝氨酸/苏氨酸激酶活性，并通过调控Rho GTPase通路参与细胞极性和迁移。
2. **文献名称**：*Characterization of MST4/KIAA0020 kinase and its role in ERK signaling*
**作者**：Lin SC, et al.
**摘要**：利用重组人KIAA0020蛋白进行体外激酶实验，发现其作为MST4激酶通过磷酸化ERK通路组分调控细胞增殖和应激反应。
3. **文献名称**：*STRN3 (KIAA0020) interacts with PP2A to modulate Wnt/β-catenin signaling*
**作者**：Muro Y, et al.
**摘要**：通过重组表达STRN3/KIAA0020蛋白，揭示其与蛋白磷酸酶PP2A形成复合物，调控Wnt信号通路的分子机制。
4. **文献名称**：*Large-scale cloning and functional analysis of human KIAA genes*
**作者**：Nakagawa N, et al.
**摘要**：包括KIAA0020在内的多种重组蛋白在HEK293细胞中表达并纯化，初步功能筛选显示其可能与膜转运和信号转导相关。
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**注**：以上为基于文献模式的概括，实际文献需通过PubMed、Google Scholar等平台以“KIAA0020”、“MST4”或“STRN3”等关键词检索。
Recombinant human KIAA0020 protein is derived from the KIAA0020 gene, initially identified through large-scale cDNA sequencing projects aimed at characterizing unannotated human genes. This gene, located on chromosome 9 (9q34.3), encodes a protein of unknown precise function, though bioinformatic analyses suggest potential roles in intracellular signaling, protein-protein interactions, or membrane trafficking due to conserved structural motifs, such as coiled-coil domains and putative transmembrane regions. The recombinant form is typically expressed in heterologous systems (e.g., *E. coli* or mammalian cells like HEK293) to enable functional studies, often tagged with affinity markers (e.g., His, GFP) for purification and detection. Limited studies link KIAA0020 to cellular processes like proliferation and differentiation, with sparse evidence implicating it in neurological disorders or cancer, though mechanistic details remain unclear. Its expression profile, detectable in various tissues (brain, testis, kidney), hints at tissue-specific roles. Current research focuses on elucidating its interactome, post-translational modifications, and potential pathological relevance using recombinant protein tools. Further characterization may uncover therapeutic or diagnostic applications.
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