| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0082 |
| Uniprot No | Q8N1G2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-835aa |
| 活性数据 | MKRRTDPECTAPIKKQKKRVAELALSLSSTSDDEPPSSVSHGAKASTTSLSGSDSETEGKQHSSDSFDDAFKADSLVEGTSSRYSMYNSVSQKLMAKMGFREGEGLGKYSQGRKDIVEASSQKGRRGLGLTLRGFDQELNVDWRDEPEPSACEQVSWFPECTTEIPDTQEMSDWMVVGKRKMIIEDETEFCGEELLHSVLQCKSVFDVLDGEEMRRARTRANPYEMIRGVFFLNRAAMKMANMDFVFDRMFTNPRDSYGKPLVKDREAELLYFADVCAGPGGFSEYVLWRKKWHAKGFGMTLKGPNDFKLEDFYSASSELFEPYYGEGGIDGDGDITRPENISAFRNFVLDNTDRKGVHFLMADGGFSVEGQENLQEILSKQLLLCQFLMALSIVRTGGHFICKTFDLFTPFSVGLVYLLYCCFERVCLFKPITSRPANSERYVVCKGLKVGIDDVRDYLFAVNIKLNQLRNTDSDVNLVVPLEVIKGDHEFTDYMIRSNESHCSLQIKALAKIHAFVQDTTLSEPRQAEIRKECLRLWGIPDQARVAPSSSDPKSKFFELIQGTEIDIFSYKPTLLTSKTLEKIRPVFDYRCMVSGSEQKFLIGLGKSQIYTWDGRQSDRWIKLDLKTELPRDTLLSVEIVHELKGEGKAQRKISAIHILDVLVLNGTDVREQHFNQRIQLAEKFVKAVSKPSRPDMNPIRVKEVYRLEEMEKIFVRLEMKIIKGSSGTPKLSYTGRDDRHFVPMGLYIVRTVNEPWTMGFSKSFKKKFFYNKKTKDSTFDLPADSIAPFHICYYGRLFWEWGDGIRVHDSQKPQDQDKLSKEDVLSFIQMHRA |
| 分子量 | 121.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0082蛋白的假设性参考文献，基于可能的学术研究方向整理(若需实际文献，建议通过PubMed或Google Scholar进一步检索)：
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1. **"Cloning and Functional Characterization of Recombinant Human KIAA0082 Protein"**
*Author: Zhang et al.*
摘要：研究通过PCR克隆人KIAA0082基因，构建重组质粒并在HEK293细胞中表达。纯化后的蛋白经Western blot验证，并发现其在体外具有ATP结合活性，提示可能参与细胞能量代谢调控。
2. **"KIAA0082 Interaction with p53: Implications in Tumor Suppression"**
*Author: Tanaka et al.*
摘要：利用重组KIAA0082蛋白进行免疫共沉淀实验，揭示其与肿瘤抑制因子p53的直接相互作用。功能研究表明，KIAA0082可能通过调控p53稳定性影响癌细胞凋亡。
3. **"Structural Analysis of Recombinant KIAA0082 Reveals a Novel Protein Fold"**
*Author: Lee et al.*
摘要：通过X射线晶体学解析重组KIAA0082蛋白的3D结构，发现其含有一个独特的α-螺旋结构域，可能为核酸结合或蛋白相互作用的关键区域，为功能研究提供结构基础。
4. **"KIAA0082 Modulates DNA Damage Response in Human Cells"**
*Author: Müller et al.*
摘要：利用重组KIAA0082蛋白进行体外细胞实验，发现其过表达显著增强DNA损伤信号(γ-H2AX焦点形成)，提示其在DNA修复通路中的潜在作用。
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**注意**：上述内容为基于研究方向的假设性文献概括，实际文献需通过学术数据库检索确认。若需具体文献，建议结合KIAA0082的别名(如HSPC142)或功能关键词(如“DNA修复”“ATP酶”)扩展搜索。
**Background of Recombinant Human KIAA0082 Protein**
The KIAA0082 gene, identified through large-scale sequencing projects, encodes a protein whose biological functions remain partially characterized. Predominantly expressed in various tissues, including the brain and testes, KIAA0082 is hypothesized to participate in cellular processes such as DNA repair, RNA metabolism, or signaling pathways, though its precise mechanistic roles are under investigation. Structural analyses suggest it contains conserved domains potentially involved in protein-protein interactions or nucleic acid binding, hinting at regulatory or scaffolding functions.
Recombinant human KIAA0082 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells*)*, enabling studies on its biochemical properties and interactions. Its production facilitates *in vitro* assays, antibody development, and exploration of its association with diseases. Notably, emerging evidence links KIAA0082 dysregulation to neurodevelopmental disorders and cancer, sparking interest in its therapeutic or diagnostic potential. Despite incomplete functional mapping, recombinant KIAA0082 serves as a critical tool for unraveling its contributions to cellular homeostasis and pathology. Further research is needed to clarify its molecular partners and pathways, enhancing understanding of its role in health and disease.
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