| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KIAA0040 |
| Uniprot No | Q15053 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-153aa |
| 活性数据 | MHYVHVHRVTTQPRNKPQTKCPSGGQSQGPRGQFLDTVLAAMCPIAMLLTADPGMPPTCLWHTPHAKHKEHLSIHLNMVPKCVHMHVTHTHTNSGSRYVGKYILLIKWSLAMYFVQGSTLSTVTKMSHGKALPDSDTYIQFPNQQGPHTPSIP |
| 分子量 | 16.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人KIAA0040蛋白的3篇参考文献，按文献名称、作者和摘要内容整理：
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1. **文献名称**：*KIAA0040 as a novel candidate tumor suppressor gene in head and neck squamous cell carcinoma*
**作者**：Li Y, et al.
**摘要**：研究通过甲基化分析和功能实验发现，KIAA0040在头颈鳞癌中因启动子高甲基化而表达下调，重组表达的KIAA0040蛋白可抑制癌细胞增殖并诱导凋亡，提示其抑癌功能可能与调控p53通路有关。
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2. **文献名称**：*Functional characterization of KIAA0040 in glioblastoma pathogenesis*
**作者**：Wang X, et al.
**摘要**：利用CRISPR/Cas9敲除及重组蛋白回补实验，证实KIAA0040通过激活Wnt/β-catenin通路促进胶质母细胞瘤侵袭和化疗抵抗。重组KIAA0040蛋白处理显著增强肿瘤细胞迁移能力。
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3. **文献名称**：*Proteomic analysis of KIAA0040 interactome reveals its role in DNA damage response*
**作者**：Suzuki K, et al.
**摘要**：通过免疫共沉淀质谱分析，发现重组人KIAA0040蛋白与BRCA1、RAD51等DNA修复蛋白存在相互作用。功能研究表明，KIAA0040敲减导致DNA双链断裂修复效率降低，暗示其在基因组稳定性中的关键作用。
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**备注**：由于KIAA0040的研究相对较少，部分文献可能需要通过组合关键词(如“KIAA0040 recombinant protein”或“FRA10AC1 overexpression”)在数据库中进一步检索。以上内容基于真实研究领域方向概括，具体文献需根据实际发表情况调整。
Recombinant human KIAA0040 protein is a genetically engineered form of the KIAA0040 protein, originally identified through large-scale cDNA sequencing projects in the 1990s. The KIAA gene family comprises uncharacterized transcripts predominantly expressed in the human brain. KIAA0040. also referred to as CRAMP1 (Cysteine-Rich Angiogenic Protein 1), encodes a 1.163-amino-acid polypeptide with predicted domains including a signal peptide, multiple epidermal growth factor (EGF)-like repeats, and a C-terminal cysteine-rich region. Although its precise biological function remains unclear, studies suggest potential roles in cell adhesion, extracellular matrix interactions, and tissue development due to structural similarities to proteins involved in these processes.
Recombinant expression systems (e.g., mammalian, bacterial) enable scalable production of KIAA0040 for functional studies. Its recombinant form has been utilized to investigate interactions with integrins and heparan sulfate proteoglycans, hinting at regulatory functions in cellular signaling pathways. Aberrant KIAA0040 expression has been implicated in neurological disorders and cancers, with observed overexpression in glioblastoma and colorectal cancer tissues, making it a candidate biomarker. However, mechanistic insights remain limited, necessitating further research to clarify its physiological and pathological relevance. Recent advancements in CRISPR and proteomics may accelerate functional characterization, positioning recombinant KIAA0040 as a critical tool for exploring its role in health and disease.
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