| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IRX1 |
| Uniprot No | P78414 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-480aa |
| 活性数据 | MSFPQLGYPQYLSAAGPGAYGGERPGVLAAAAAAAAAASSGRPGAAELGGGAGAAAVTSVLGMYAAAGPYAGAPNYSAFLPYAADLSLFSQMGSQYELKDNPGVHPATFAAHTAPAYYPYGQFQYGDPGRPKNATRESTSTLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKVTWGARSKDQEDGALFGSDTEGDPEKAEDDEEIDLESIDIDKIDEHDGDQSNEDDEDKAEAPHAPAAPSALARDQGSPLAAADVLKPQDSPLGLAKEAPEPGSTRLLSPGAAAGGLQGAPHGKPKIWSLAETATSPDGAPKASPPPPAGHPGAHGPSAGAPLQHPAFLPSHGLYTCHIGKFSNWTNSAFLAQGSLLNMRSFLGVGAPHAAPHGPHLPAPPPPQPPVAIAPGALNGDKASVRSSPTLPERDLVPRPDSPAQQLKSPFQPVRDNSLAPQEGTPRILAALPSA |
| 分子量 | 52.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IRX1蛋白的示例参考文献(基于常见研究方向模拟，建议通过学术数据库核实最新文献)：
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1. **"Cloning and Expression of Human IRX1 Gene in Escherichia coli for Functional Studies"**
*作者：Zhang L, et al.*
**摘要**：研究报道了人IRX1基因的克隆及其在大肠杆菌中的重组表达，通过优化诱导条件获得可溶性蛋白，并利用Western blot验证表达特异性，为后续功能研究提供基础。
2. **"IRX1 Suppresses Tumor Metastasis via Epigenetic Regulation of EMT Markers in Gastric Cancer"**
*作者：Wang Y, et al.*
**摘要**：探讨重组IRX1蛋白在胃癌中的抑癌作用，证明其通过调控上皮-间质转化(EMT)相关基因的甲基化抑制肿瘤转移，为癌症治疗提供潜在靶点。
3. **"Structural Characterization of Recombinant Human IRX1 Protein and Its DNA-Binding Properties"**
*作者：Kimura T, et al.*
**摘要**：利用X射线晶体学解析IRX1蛋白结构，揭示其同源结构域与靶DNA结合的关键位点，阐明其在基因转录调控中的分子机制。
4. **"IRX1 Modulates Cardiac Development through Interaction with TBX5 in Zebrafish Models"**
*作者：Chen H, et al.*
**摘要**：通过斑马鱼模型发现重组IRX1蛋白与TBX5协同调控心脏发育，基因敲除实验证实其异常表达导致心脏形态缺陷。
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**说明**：
- 以上文献名称与摘要为模拟内容，实际研究中IRX1多与发育调控(如心脏、神经系统)和肿瘤抑制相关。
- 建议通过**PubMed**或**Web of Science**搜索关键词“recombinant human IRX1 protein”或“IRX1 gene function”获取真实文献。
- 注意IRX1可能与不同信号通路(如Wnt、BMP)存在关联，可根据具体研究方向筛选文献。
Recombinant human IRX1 protein is derived from the Iroquois homeobox gene family (IRX), which encodes transcription regulators critical in embryonic development and tissue patterning. IRX1. located on chromosome 5p15.3. contains a conserved homeodomain that binds DNA and a distinct IRO motif involved in protein interactions. It plays roles in cell differentiation, organogenesis (particularly in the nervous system and heart), and maintains tissue polarity. Dysregulation of IRX1 is linked to cancers, including gastric and lung cancers, where it often acts as a tumor suppressor. Epigenetic silencing via promoter hypermethylation and histone modifications leads to its downregulation in malignancies, correlating with poor prognosis. Recombinant IRX1 is typically produced in bacterial (e.g., E. coli) or mammalian expression systems, enabling studies on its DNA-binding properties, regulatory networks, and potential therapeutic applications. Its structural integrity (verified via Western blot, mass spectrometry) ensures functionality in experimental models exploring developmental biology and cancer mechanisms. Research focuses on leveraging recombinant IRX1 to restore normal gene expression patterns in diseased tissues or as a biomarker for early cancer detection.
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