| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IQCK |
| Uniprot No | Q8N0W5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-287aa |
| 活性数据 | MAAPRQIPSHIVRLKPSCSTDSSFTRTPVPTVSLASRELPVSSWQVTEPSSKNLWEQICKEYEAEQPPFPEGYKVKQEPVITVAPVEEMLFHGFSAEHYFPVSHFTMISRTPCPQDKSETINPKTCSPKEYLETFIFPVLLPGMASLLHQAKKEKCFERKRTKFIACDFLTEWLYNQNPKRAGEPFTEFFSIPFVEERLKQHPRPPIPLSLLLTEEEAALYIQSFWRACVVRCDPEIQELRQWQKKLREAKHIHQQVKIFWAKQEQKVKCKMEDDAVPAAKMKIPSS |
| 分子量 | 59.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IQCK蛋白的3篇文献示例，涵盖不同研究方向：
1. **文献名称**: *"Expression and Purification of Recombinant Human IQCK Protein in Escherichia coli"*
**作者**: Smith et al.
**摘要**: 报道了在大肠杆菌中高效表达重组人IQCK蛋白的方法，通过亲和层析纯化获得高纯度蛋白，并验证其二级结构完整性，为后续功能研究提供基础。
2. **文献名称**: *"Functional Analysis of IQCK in Ciliogenesis Using Recombinant Protein Complementation"*
**作者**: Zhang et al.
**摘要**: 利用重组人IQCK蛋白回复实验，证明其在纤毛形成中的关键作用，发现IQCK通过与钙调蛋白互作调控纤毛组装相关信号通路。
3. **文献名称**: *"Structural Insights into Human IQCK by Cryo-EM: Implications for IQ Motif-Mediated Protein Interactions"*
**作者**: Tanaka et al.
**摘要**: 首次解析重组人IQCK蛋白的冷冻电镜结构，揭示IQ基序介导的钙调蛋白结合机制，为疾病相关突变位点提供分子解释。
*注：上述文献为示例，若需实际引用，请通过学术数据库(如PubMed)检索最新研究。部分研究可能集中于IQCK旁系同源物(如IQCG)，建议结合具体研究方向扩展关键词。*
Human IQCK (IQ motif containing K) is a calcium/calmodulin-binding protein belonging to the IQ motif-containing protein family. It is encoded by the *IQCK* gene located on chromosome 17q21.32 and is evolutionarily conserved, indicating its functional significance. The protein contains multiple IQ motifs, which mediate interactions with calmodulin and other calcium-signaling molecules, as well as predicted phosphorylation sites, suggesting roles in signal transduction. IQCK is predominantly expressed in tissues with high ciliary activity, such as the testis, brain, and respiratory epithelium, implicating its involvement in cilia-related processes. Studies link IQCK to the regulation of ciliogenesis, cell motility, and cytoskeletal dynamics. Dysregulation of IQCK has been associated with ciliopathies, neurological disorders, and infertility.
Recombinant human IQCK protein is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies. Its recombinant form allows researchers to investigate molecular interactions, structural properties, and enzymatic activities in vitro. Applications include elucidating calcium-dependent signaling pathways, exploring cilia-associated disease mechanisms, and screening therapeutic compounds. Purification often involves affinity tags (e.g., His-tag) and chromatographic techniques, ensuring high specificity and yield. Ongoing research aims to clarify its precise biological roles and therapeutic potential in cilia-related pathologies.
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