| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IRGC |
| Uniprot No | Q6NXR0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-463aa |
| 活性数据 | MATSKLPVVPGEEENTILMAKERLEALRTAFESGDLPQAASHLQELLASTESIRLEVGVTGESGAGKSSLINALRGLEAEDPGAALTGVMETTMQPSPYPHPQFPDVTLWDLPGAGSPGCPADKYLKQVDFSRYDFFLLVSPRRCGAVETRLAAEILCQGKKFYFVRTKVDEDLAATRTQRPSGFREAAVLQEIRDHCAERLREAGVADPRIFLVSNLSPARYDFPTLVSTWEHDLPSHRRHAGLLSLPDISLEALQKKKAMLQEQVLKTALVLGVIQALPVPGLAAAYDDALLIHSLRGYHRSFGLDDDSLAKLAEQVGKQAGDLRSVIRSPLANEVSPETVLRLYSQSSDGAMRVARAFERGIPVFGTLVAGGISFGAVYTMLQGCLNEMAEDAQRVRIKALEDDEPQPEVSLEVASDNGVEKGGSGEGGGEEAPLSTCRKLGLLLKYILDSWKKHDSEEK |
| 分子量 | 76.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IRGC蛋白的假设性参考文献示例(请注意，以下内容为模拟创作，实际文献可能需要进一步检索验证)：
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1. **《Recombinant human IRGC protein induces antiviral responses in macrophages》**
*Author: Smith et al. (2020)*
摘要：本研究通过在大肠杆菌中表达并纯化重组人IRGC蛋白，证明其能够激活巨噬细胞的干扰素信号通路，显著抑制RNA病毒复制，提示IRGC在天然免疫中的潜在作用。
2. **《IRGC-mediated autophagy regulation in inflammatory diseases》**
*Author: Lee & Kim (2018)*
摘要：利用重组人IRGC蛋白进行体外实验，发现其通过结合自噬关键蛋白LC3.调控细胞自噬水平，可能为炎症性肠病的治疗提供新靶点。
3. **《Structural characterization of IRGC and its interaction with TLR4》**
*Author: Zhang et al. (2021)*
摘要：通过重组IRGC蛋白的晶体结构解析，揭示其与Toll样受体4(TLR4)的相互作用机制，为理解IRGC在先天免疫中的信号传导提供结构基础。
4. **《Recombinant IRGC as a biomarker in colorectal cancer》**
*Author: Patel et al. (2019)*
摘要：研究发现，重组IRGC蛋白在结直肠癌患者血清中表达异常，可能作为早期诊断的生物标志物，并体外验证其抑制肿瘤细胞迁移的功能。
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**注意**：以上文献为示例性内容，若需真实参考文献，建议通过学术数据库(如PubMed、Web of Science)以“recombinant human IRGC protein”或相关关键词检索。若IRGC为特定领域缩写(如免疫相关基因)，需结合具体研究方向调整检索策略。
Recombinant human IRGC (Immunity-Related GTPase Family M) protein is a key player in innate immunity, particularly in host defense against intracellular pathogens. Belonging to the IRG protein family, IRGC is induced by interferon-gamma (IFN-γ) and regulates cell-autonomous immune responses. These GTPases orchestrate the destruction of pathogen-containing vacuoles, enabling lysosomal clearance of microbes like *Mycobacterium tuberculosis* or *Toxoplasma gondii*. Structurally, IRGC contains a conserved GTP-binding domain and oligomerization motifs, which facilitate its membrane-targeting activity and pathogen sensing.
The recombinant form of human IRGC is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies and therapeutic exploration. Its production enables research into molecular mechanisms of pathogen resistance, autophagy, and inflammatory signaling. Aberrant IRGC expression has been linked to autoimmune disorders and chronic infections, highlighting its clinical relevance. However, challenges persist in achieving proper protein folding and post-translational modifications in vitro, which are critical for functional studies. Current applications include drug screening, elucidating host-pathogen interactions, and developing targeted immunotherapies. Advances in recombinant IRGC technology continue to bridge gaps in understanding cellular immunity and designing precision antimicrobial strategies.
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