| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IQUB |
| Uniprot No | Q8NA54 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-791aa |
| 活性数据 | MSNQQEKYEAQNIVNSTEESDDAFDTVTIPVPSEEPQESDQTEEHESGIEQFSESHAIHVEEQSDQSFSSLEPDNEQLMEEVISPRQVSYTPQHHEKQYAMQRPNDDSLAFLDKIKSVKESLQESMEDSLATVKVVLIPVGQEIVIPFKVDTILKYLKDHFSHLLGIPHSVLQIRYSGKILKNNETLVQHGVKPQEIVQVEIFSTNPDLYPVRRIDGLTDVSQIITVTVQTGLDQYQQVPVEIVKSDFHKPFLGGFRHKVTGVEYHNAGTQTVPKRIPERLSIFCRDTQTVFQKKNLQQTTNTTSTQMTNIGVYVSNMTDKLVTPGKYFSAAEYHAQRLKAVIVIQTYYRQWHAKIFVENLRRQKSLRLEWETQQELRKIREKEEWIKLDYHRRHNPKTNEDFEFLYNALEFWRQEELTRINQSFTGAERKAALCELLEKETQIIASIGRHRYIAYMANQEAAIQAFLDKCSAPKIWRTPNGKTIEMDTQFTIRARELQNIYKCIMLKNISQDERLDVLLTLKHTVKEHECKLTQEILELIDREVDLMMRGVKHHNLEGLRKRIATLFFHYIKTPLFNPEVAKYLKVPQDPLKFYKKIYFCHSCQLYLPSTEFSVSSTSRRIYRCRNCINLQNEAQKRESFLKYKCLLQQLYYTEADYEDDSKIAFLMQLQDIQYLTENIWASQSVLSACDNLSDLVMVRWNKSLEWSPWNCILLTKDEAAAHLKLTSIEEGYERSFIHKIKHKHILAKNYFSQVPVLASFILDDGEIDEIRWKYHSDTTPKIIESQRPPH |
| 分子量 | 119 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于“IQUB蛋白”可能为拼写错误或简称(如正确名称为IQ motif-containing protein或相关家族成员)，以下提供3篇假设性重组人IQ结构域蛋白的参考范文，实际文献需通过学术数据库核实：
1. **文献名称**: "Expression and functional characterization of human IQUB, a calmodulin-binding protein involved in sperm motility"
**作者**: Liu Y, et al.
**摘要**: 本研究在大肠杆菌中成功表达了重组人IQUB蛋白，证实其与钙调蛋白(Calmodulin)的相互作用，并发现其在调节精子细胞钙信号通路及运动功能中的关键作用。纯化蛋白通过体外实验展示了钙离子依赖性构象变化。
2. **文献名称**: "Structural insights into the IQ motif of IQUB protein and its role in neuronal development"
**作者**: Chen X, et al.
**摘要**: 通过X射线晶体学解析重组人IQUB蛋白的IQ结构域三维结构，揭示了其与微管结合蛋白Tau的相互作用位点，提示其在神经元轴突生长和神经发育疾病中的潜在调控机制。
3. **文献名称**: "High-yield production of recombinant human IQUB in mammalian cells and its phosphorylation analysis"
**作者**: Tanaka K, et al.
**摘要**: 开发了HEK293细胞表达系统，实现IQUB蛋白的哺乳动物特异性糖基化修饰，并通过质谱鉴定了CDK5激酶介导的Ser-15磷酸化位点，为研究其在中枢神经系统中的功能提供工具。
**注意事项**：
- 上述文献为模拟示例，IQUB可能指IQCG(精子活化相关蛋白)或类似蛋白，建议通过UniProt或PubMed核对基因名(如：Homo sapiens IQUB, Gene ID: 340527)。
- 实际研究可查询关键词：recombinant human IQUB/IQ motif protein/calmodulin-binding protein expression.
**Background of Recomcombinant Human IQUB Protein**
The IQUB protein, encoded by the *IQUB* gene in humans, is a relatively understudied protein implicated in cellular signaling and structural regulation. While its precise biological functions remain under investigation, in silico analyses suggest it contains multiple IQ motifs, which are known to mediate interactions with calmodulin and other calcium-sensitive proteins. This indicates potential roles in calcium-dependent signaling pathways, possibly influencing cytoskeletal organization, intracellular trafficking, or synaptic regulation.
Interest in recombinant human IQUB stems from its potential involvement in neurological and developmental processes. Studies in model organisms have linked IQUB homologs to ciliary function and neural development, suggesting evolutionary conservation. Recombinant production of IQUB enables researchers to explore its biochemical properties, interaction partners, and mechanisms in vitro. Typically expressed in *E. coli* or mammalian systems with affinity tags (e.g., His-tag), the purified protein facilitates structural studies (e.g., X-ray crystallography) and functional assays (e.g., binding affinity measurements).
Current research aims to clarify its physiological relevance, including possible associations with ciliopathies or neurodevelopmental disorders. Additionally, recombinant IQUB serves as a tool for antibody development and pathway screening in drug discovery. Despite progress, further validation of its in vivo roles and disease linkages is needed to unlock its therapeutic or diagnostic potential.
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