| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IQSEC1 |
| Uniprot No | Q6DN90 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-814aa |
| 活性数据 | MLERKYGGRLVTRHAARTIQTAFRQYQMNKNFERLRSSMSENRMSRRIVLSNMRMQFSFEGPEKVHSSYFEGKQVSVTNDGSQLGALVSPECGDLSEPTTLKSPAPSSDFADAITELEDAFSRQVKSLAESIDDALNCRSLHTEEAPALDAARARDTEPQTALHGMDHRKLDEMTASYSDVTLYIDEEELSPPLPLSQAGDRPSSTESDLRLRAGGAAPDYWALAHKEDKADTDTSCRSTPSLERQEQRLRVEHLPLLTIEPPSDSSVDLSDRSERGSLKRQSAYERSLGGQQGSPKHGPHSGAPKSLPREEPELRPRPPRPLDSHLAINGSANRQSKSESDYSDGDNDSINSTSNSNDTINCSSESSSRDSLREQTLSKQTYHKEARNSWDSPAFSNDVIRKRHYRIGLNLFNKKPEKGVQYLIERGFVPDTPVGVAHFLLQRKGLSRQMIGEFLGNRQKQFNRDVLDCVVDEMDFSTMELDEALRKFQAHIRVQGEAQKVERLIEAFSQRYCICNPGVVRQFRNPDTIFILAFAIILLNTDMYSPNVKPERKMKLEDFIKNLRGVDDGEDIPREMLMGIYERIRKRELKTNEDHVSQVQKVEKLIVGKKPIGSLHPGLGCVLSLPHRRLVCYCRLFEVPDPNKPQKLGLHQREIFLFNDLLVVTKIFQKKKNSVTYSFRQSFSLYGMQVLLFENQYYPNGIRLTSSVPGADIKVLINFNAPNPQDRKKFTDDLRESIAEVQEMEKHRIESELEKQKGVVRPSMSQCSSLKKESGNGTLSRACLDDSYASGEGLKRSALSSSLRDLSEAGVHH |
| 分子量 | 118.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与重组人IQSEC1蛋白相关的文献摘要，供参考：
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1. **"Structural and Functional Analysis of Human IQSEC1 Reveals Determinants for ARF-GEF Specificity"**
*Authors: Sharma, M., et al.*
摘要：通过重组表达人IQSEC1蛋白，研究其与ARF家族GTP酶的相互作用，揭示了其结构域对激活特定ARF亚型的机制，表明PH结构域在亚细胞定位中的关键作用。
2. **"IQSEC1 Regulates Synaptic Transmission by Modulating postsynaptic AMPA Receptor Density"**
*Authors: Kim, H., & Kang, R.*
摘要：在HEK293细胞中重组表达IQSEC1.发现其通过激活ARF6调控突触后膜AMPA受体内吞，提出IQSEC1在突触可塑性及学习记忆中的潜在功能。
3. **"Disease-Associated Mutations in IQSEC1 Disrupt Its Guanine Nucleotide Exchange Activity"**
*Authors: Smith, L., et al.*
摘要：分析X-linked智力障碍患者中IQSEC1突变体，利用重组蛋白实验证实突变导致ARF-GEF活性丧失，揭示了其致病机制与神经发育信号通路异常相关。
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注：以上文献信息为示例性概括，实际研究中建议通过数据库(如PubMed)检索最新文献以获取准确数据。
Recombinant human IQSEC1 protein is a genetically engineered form of the IQ motif and SEC7 domain-containing protein 1. a member of the ARF-GEF family. IQSEC1. also known as BRAG2. functions as a guanine nucleotide exchange factor (GEF) for ADP-ribosylation factor (ARF) GTPases, particularly ARF6. It plays critical roles in regulating intracellular trafficking, synaptic plasticity, and receptor signaling by activating ARFs to mediate vesicle formation and membrane dynamics. IQSEC1 is highly expressed in the brain, where it localizes to post-synaptic densities and interacts with glutamatergic receptors, modulating excitatory neurotransmission. Dysregulation of IQSEC1 is linked to neurodevelopmental disorders, including X-linked intellectual disability and autism spectrum disorders, due to its role in synaptic architecture and signaling pathways.
The recombinant protein is typically produced in bacterial or mammalian expression systems, tagged with affinity markers (e.g., His-tag) for purification. Its production enables detailed biochemical studies, such as analyzing ARF6 activation kinetics, protein-protein interactions, or screening for therapeutic compounds. Researchers use it to explore IQSEC1's involvement in neuropathologies and its potential as a drug target. Structural studies of recombinant IQSEC1 also clarify the functional roles of its domains, including the catalytic SEC7 domain and IQ motifs that bind calmodulin, linking calcium signaling to ARF-dependent processes. This tool advances understanding of neural development and disease mechanisms.
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