| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IQGAP3 |
| Uniprot No | Q86VI3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 602-698aa |
| 活性数据 | DTNTAQRMALGVAAINQAIKEGKAAQTERVLRNPAVALRGVVPDCANGYQRALESAMAKKQRPADTAFWVQHDMKDGTAYYFHLQTFQGIWEQPPGC |
| 分子量 | 36.41 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IQGAP3蛋白的假设性参考文献示例(实际文献需通过数据库验证)：
1. **文献名称**："IQGAP3 Regulates Cell Proliferation and Apoptosis via the RAS/ERK Pathway"
**作者**：Smith A, et al.
**摘要**：研究揭示了重组人IQGAP3蛋白在调控细胞周期和RAS/ERK信号通路中的作用，通过体外实验证明其过表达可促进癌细胞增殖并抑制凋亡。
2. **文献名称**："Expression and Functional Analysis of Recombinant Human IQGAP3 in Epithelial Cell Migration"
**作者**：Chen L, et al.
**摘要**：报道了在大肠杆菌系统中成功表达重组人IQGAP3蛋白，并发现其通过与细胞骨架蛋白相互作用增强上皮细胞的定向迁移能力。
3. **文献名称**："IQGAP3 as a Novel Biomarker in Colorectal Cancer: Role in Tumor Invasion"
**作者**：Tanaka K, et al.
**摘要**：利用重组IQGAP3蛋白进行功能研究，证实其通过激活Wnt/β-catenin通路促进结直肠癌细胞侵袭，提示其作为癌症治疗靶点的潜力。
4. **文献名称**："Structural Characterization of Recombinant Human IQGAP3 and Its Interaction with CDC42"
**作者**：Wang Y, et al.
**摘要**：通过X射线晶体学解析重组人IQGAP3蛋白结构，并阐明其与CDC42蛋白的互作机制，为理解IQGAP家族蛋白的调控网络提供依据。
**注**：以上文献为示例，实际引用时请通过PubMed、Web of Science等平台搜索真实文献(关键词：recombinant human IQGAP3、IQGAP3 function)。
Recombinant human IQGAP3 protein is a engineered form of the naturally occurring IQ motif-containing GTPase-activating protein 3. which belongs to the IQGAP family of scaffolding proteins. Structurally, IQGAP3 contains conserved domains including an actin-binding calponin homology domain, a GTPase-activating protein (GAP)-related domain, and multiple IQ motifs that interact with calmodulin and other calcium-binding proteins. It functions as a molecular hub coordinating cytoskeletal dynamics, cell-cell adhesion, and mitotic processes by interacting with partners such as Rac1. Cdc42. actin filaments, and components of the cell division machinery.
Studies suggest IQGAP3 is highly expressed in proliferating tissues and cancer cells, where it promotes cell cycle progression, cytokinesis, and epithelial-mesenchymal transition. Its overexpression correlates with poor prognosis in multiple cancers, implicating it in tumorigenesis and metastasis. Recombinant IQGAP3 enables functional studies through in vitro biochemical assays and structural analysis. It is typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications. Ongoing research explores its diagnostic potential as a cancer biomarker and therapeutic target. Compared to IQGAP1/2. IQGAP3 shows distinct spatiotemporal expression patterns during cell division, highlighting functional specialization within the IQGAP family.
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