| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IQCF1 |
| Uniprot No | Q8N6M8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-205aa |
| 活性数据 | MEEKQPQKTKEPSKEDEPQQKEMPTHLSLGAESKAEAKTPVLVETQTVDNANEKSEKPPENQKKLSDKDTVATKIKAWWRGTLVRRALLHAALSACIIQCWWRLILSKILKKRRQAALEAFSRKEWAAVTLQSQARMWRIRRRYCQVLNAVRIIQAYWRCRSCASRGFIKGQYRVTANQLHLQLEILLDSGPCIVTECIPFSIKE |
| 分子量 | 50.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IQCF1蛋白的假设性参考文献示例(请注意，以下内容为模拟生成，实际文献可能需要通过学术数据库核实)：
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1. **文献名称**：*Expression and Purification of Recombinant Human IQCF1 in Escherichia coli*
**作者**：Zhang L. et al.
**摘要**：该研究报道了利用大肠杆菌表达系统高效表达可溶性人IQCF1蛋白的优化方法，并通过亲和层析技术纯化获得高纯度蛋白，验证了其与钙调蛋白的相互作用。
2. **文献名称**：*Structural Insights into Human IQCF1 by Cryo-EM*
**作者**：Wang Y. et al.
**摘要**：通过冷冻电镜解析了重组人IQCF1的三维结构，揭示其IQ结构域与微管结合的关键区域，为研究其在细胞骨架调控中的作用提供结构基础。
3. **文献名称**：*Role of IQCF1 in Spermiogenesis: Knockout Mouse Model Analysis*
**作者**：Chen H. et al.
**摘要**：利用重组人IQCF1蛋白进行体外功能补偿实验，发现其在精子形成中调控鞭毛组装，IQCF1缺失导致雄性小鼠不育。
4. **文献名称**：*IQCF1 Interacts with Calmodulin in a Calcium-Dependent Manner*
**作者**：Smith J. et al.
**摘要**：通过表面等离子共振技术证实重组人IQCF1的钙依赖性结合特性，揭示了其在细胞钙信号通路中的潜在调节作用。
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**注意**：以上为模拟文献，实际研究需参考真实学术数据库(如PubMed、Web of Science)。建议通过关键词检索获取准确信息。
Recombinant human IQCF1 (IQ motif-containing F1) protein is a genetically engineered variant of the naturally occurring IQCF1 protein, designed for experimental and therapeutic applications. IQCF1 is a member of the CTAGE (cutaneous T-cell lymphoma-associated antigen) family, characterized by conserved IQ motifs that mediate interactions with calmodulin and other calcium-binding proteins. These motifs suggest its potential role in calcium-dependent signaling pathways, though its precise molecular functions remain under investigation.
Studies indicate that IQCF1 is predominantly expressed in the testis, implying a possible role in spermatogenesis or male reproductive biology. It may participate in acrosome formation or sperm-egg fusion, given its structural similarities to other fertilization-related proteins. Recombinant IQCF1 is typically produced in bacterial or mammalian expression systems, enabling high-purity yields for functional studies. Its recombinant form facilitates antibody development, protein-protein interaction assays, and mechanistic exploration of its biological roles.
Current research focuses on IQCF1's association with infertility and its potential as a diagnostic biomarker. Additionally, structural analysis of recombinant IQCF1 aids in mapping functional domains and calcium-responsive regions. Despite limited clinical data, its testis-specific expression and conserved motifs make it a compelling target for reproductive health research. Further studies are needed to elucidate its physiological significance and therapeutic relevance.
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