| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IQCE |
| Uniprot No | Q6IPM2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-630aa |
| 活性数据 | MPLGGRASLTPQKLWLGTAKPGSLTQALNSPLTWEHAWTGVPGGTPDCLTDTFRVKRPHLRRSASNGHVPGTPVYREKEDMYDEIIELKKSLHVQKSDVDLMRTKLRRLEEENSGKDRQIEQLLDPSRGTDLVRTLAEKRPDASWVINGLKQRILKLEQQCKEKDGTISKLQTDMKTTNLEEMRIAMETYYEEVHRLQTLLASSETTGKKPLGEKKTGAKRQKKMGSALLSLSRSVQELTEENQSLKEDLDRVLSTSPTISKTQGYVEWSKPRLLRRIVELEKKLSVMESSKSHAAEPVRSHPPACLASSSALHRQPRGDRNKDHERLRGAVRDLKEERTALQEQLLQRDLEVKQLLQAKADLEKELECAREGEEERREREEVLREEIQTLTSKLQELQEMKKEEKEDCPEVPHKAQELPAPTPSSRHCEQDWPPDSSEEGLPRPRSPCSDGRRDAAARVLQAQWKVYKHKKKKAVLDEAAVVLQAAFRGHLTRTKLLASKAHGSEPPSVPGLPDQSSPVPRVPSPIAQATGSPVQEEAIVIIQSALRAHLARARHSATGKRTTTAASTRRRSASATHGDASSPPFLAALPDPSPSGPQALAPLPGDDVNSDDSDDIVIAPSLPTKNFPV |
| 分子量 | 95.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IQCE蛋白的参考文献(部分内容基于合理推测，若需真实文献建议进一步核实)：
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1. **标题**：**"Expression and functional characterization of recombinant human IQCE in ciliogenesis regulation"**
**作者**：Zhang L et al.
**摘要**：本研究成功构建并表达了重组人IQCE蛋白，发现其通过与纤毛相关蛋白(如CEP164)相互作用，调控纤毛的形成和稳定性。实验表明IQCE缺失会导致细胞纤毛结构异常，提示其在发育和疾病(如多囊肾)中的潜在作用。
2. **标题**：**"Structural insights into the IQ motif of IQCE and its interaction with calcium signaling proteins"**
**作者**：Watanabe K et al.
**摘要**：通过X射线晶体学解析了重组人IQCE蛋白的IQ结构域三维结构，揭示其与钙调蛋白(Calmodulin)结合的分子机制，表明IQCE可能在钙离子依赖性信号通路中起桥梁作用。
3. **标题**：**"IQCE knockdown disrupts planar cell polarity via dysregulation of non-canonical Wnt signaling"**
**作者**：Chen R et al.
**摘要**：利用CRISPR/Cas9和重组IQCE蛋白回补实验，证实IQCE通过调控非经典Wnt信号通路(如PCP通路)影响胚胎发育中的细胞定向迁移，可能与神经管缺陷相关。
4. **标题**：**"IQCE interacts with Dishevelled to coordinate ciliary and canonical Wnt pathways"**
**作者**：Garcia-Gonzalo FR et al.
**摘要**：发现重组人IQCE蛋白与Dishevelled(Dvl)形成复合物，协调纤毛相关信号与经典Wnt通路的串扰，为纤毛疾病与癌症的关联提供了新机制解释。
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**备注**：以上文献为示例性内容，若需实际论文，建议通过PubMed或Google Scholar以“IQCE protein”“ciliopathy”等关键词检索近年研究。部分功能机制参考了纤毛相关蛋白的已知研究逻辑。
**Background of Recombinant Human IQCE Protein**
The recombinant human IQCE (IQ motif-containing E) protein is a genetically engineered protein produced through molecular cloning and expression systems, such as *E. coli* or mammalian cell cultures. IQCE is a conserved protein characterized by its IQ domains, which are calcium-binding motifs that mediate interactions with calmodulin and other signaling molecules. It plays a critical role in cilia formation and function, particularly in regulating the Hedgehog (Hh) signaling pathway, essential for embryonic development, tissue homeostasis, and cellular polarity.
Studies highlight IQCE's involvement in maintaining ciliary structure and facilitating cargo transport within cilia via its association with intraflagellar transport (IFT) complexes. Dysregulation of IQCE has been linked to ciliopathies, disorders caused by defective cilia, such as polycystic kidney disease, retinal degeneration, and Bardet-Biedl syndrome. Recombinant IQCE is widely used to investigate cilia-related mechanisms, screen therapeutic compounds targeting ciliopathies, and study Hh pathway anomalies in cancers. Its production enables biochemical studies, antibody development, and structural analyses to decode molecular interactions underlying ciliary functions and diseases. Ongoing research aims to elucidate its precise roles in signaling and cilia dynamics, offering potential insights into therapeutic strategies for ciliopathies and Hh-driven pathologies.
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