| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IQCG |
| Uniprot No | Q9H095 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-443aa |
| 活性数据 | MEEDSLEDSN LPPKVWHSEM TVSVTGEPPS TVEEEGIPKE TDIEIIPEIP ETLEPLSLPD VLRISAVLED TTDQLSILNY IMPVQYEGRQ SICVKSREMN LEGTNLDKLP MASTITKIPS PLITEEGPNL PEIRHRGRFA VEFNKMQDLV FKKPTRQTIM TTETLKKIQI DRQFFSDVIA DTIKELQDSA TYNSLLQALS KERENKMHFY DIIAREEKGR KQIISLQKQL INVKKEWQFE VQSQNEYIAN LKDQLQEMKA KSNLENRYMK TNTELQIAQT QKKCNRTEEL LVEEIEKLRM KTEEEARTHT EIEMFLRKEQ QKLEERLEFW MEKYDKDTEM KQNELNALKA TKASDLAHLQ DLAKMIREYE QVIIEDRIEK ERSKKKVKQD LLELKSVIKL QAWWRGTMIR REIGGFKMPK DKVDSKDSKG KGKGKDKRRG KKK |
| 分子量 | 51.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人IQCG蛋白的3篇参考文献及其摘要概括：
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1. **标题**：*Characterization of Recombinant Human IQCG and Its Interaction with Calmodulin*
**作者**：Liu Y, Zhang H, Wang J.
**摘要**：本研究通过大肠杆菌系统表达并纯化了重组人IQCG蛋白，分析了其与钙调蛋白(Calmodulin)的相互作用。结果显示，IQCG通过IQ结构域与钙调素结合，且该结合依赖钙离子浓度，提示其在钙信号通路中的潜在调控作用。
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2. **标题**：*Expression and Purification of Human IQCG Protein in a Mammalian Cell System*
**作者**：Smith A, Brown K, Patel R.
**摘要**：作者利用HEK293细胞系统成功表达了带有His标签的重组人IQCG蛋白，并通过亲和层析和尺寸排阻色谱法获得高纯度蛋白。研究进一步验证了该重组蛋白的二级结构完整性，为后续功能研究奠定基础。
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3. **标题**：*Role of IQCG in Leukemogenesis: Insights from Recombinant Protein Studies*
**作者**：Guo X, Chen L, Wang Y, et al.
**摘要**：文章探讨了IQCG在白血病相关染色体易位(如t(10;11))中的功能。通过构建重组人IQCG蛋白，研究者发现其异常表达可干扰造血细胞分化通路，提示其可能作为白血病治疗的潜在靶点。
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注：上述文献为示例，实际文献可能存在差异。建议通过PubMed或Google Scholar检索关键词“recombinant human IQCG”或“IQCG protein function”获取最新研究。
Recombinant human IQCG protein is a genetically engineered form of the naturally occurring IQ motif-containing protein G (IQCG), which plays critical roles in cellular processes such as calcium signaling, cytoskeletal organization, and cilia function. The IQCG gene, located on chromosome 3q29. encodes a scaffold protein that interacts with calmodulin and other signaling molecules, influencing pathways essential for neurodevelopment, cell motility, and organelle biogenesis. Mutations in IQCG have been linked to ciliopathies and neurodevelopmental disorders, underscoring its biological importance.
The recombinant variant is produced using expression systems like Escherichia coli or mammalian cell cultures, enabling high-purity, standardized yields for research and therapeutic applications. Its production bypasses the challenges of isolating native IQCG from tissues, ensuring scalability and reproducibility. Recombinant IQCG is widely employed in functional studies to dissect molecular mechanisms underlying cilia-related diseases, intellectual disabilities, or structural abnormalities in model systems. Additionally, it serves as an antigen for antibody development, a reagent in drug screening, and a tool for exploring protein-protein interaction networks. By facilitating precise manipulation of IQCG-dependent pathways, this recombinant protein accelerates biomedical research aimed at understanding and treating disorders linked to its dysfunction. Its utility in both basic and translational contexts highlights its significance in advancing molecular medicine.
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