| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IQCH |
| Uniprot No | Q86VS3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1027aa |
| 活性数据 | MAQNTENHDPVGSILIQIHEDLYQLKEKLTKFSPEEKGETLDIQSLETAIKRTEVGLRIHIEKYLNVVNQNVLTTSVNDESLYTPQASKWLLPTVIDQKSFIFPQESEGTFWQPQRQHSSSLPVFPRAKIKVSKLIKGSNISSLTVLPSSHCTDPYFTPIPVLQADAHKGILSMIERGLIPPTARITFQNPPITPRAAPLHSFDEARKIPTVATFTIPREPPPSPAEVKFFPKKQRSKGKSRRSRGHHDRKAMKVKTPLRALKSLWDYDFLIYDGVIDNTAPDFLAFKEHFSLAWGGIFSLLEHVEKFLRNYAIPEVKIKGNNLVALLPEFELTNKLTRYDLLSVLEDPAHVQMLINLPGQRYKGQDGNSEAAMKIQATWKCYKARKFFLFYRQQKWASGVIAIAWLLYCHKTRLKKILKESRQRHLENFRIRAKHLAANWNRIRTSRRTIIHIPSLGYSQPVREHIADFNTQQNMQLGRLCDILDANVNVIYICSHHMNDELVLYYKKILSLHAAVKSGNLEDRSDLQDRFKIITPEAVNIFPKHHMCLATHLMYSPKAIKRIKNLIRGTEAYIVSGLLHRDDLAVADMLDIPILGSEPELAHLYSTKSGGKRVFDSANVAVPPGIYDIYSQQQMIEQLSQLITDHLQIQRWLFKMDSEFRGNGTAFCDIPSYLKCYKWVLKESSRYGLEDWRKKWAQEPALVKISEELAGILAQHAQPVNEKRFPTWRKFLQTFLSQGGVIEAFPPADNVTNLTVDMLIEPNGKISVLSTGDQLHAESPFISSGTTVPQTSVDPQVLTYLCLQIGKACRMRDVVGYFSIDLVTFIDPSTLEQQVWATGLNLAYSDQLALTQLTLYLTNGHLDCSLSTLEVPRFVPKERKKTKCMSALSMPMLATSRYAVMTTQLRHSNLSLVFHYVFLQICRAHGIGYDLEERQGTVFILYEHLKRHKLGMLTIGEDLQGVLMTFARHLFIIHQEISAPNMQGETNFKTTIADIETILRVTKENKMRFEEEQQSKDDKNLSKPKK |
| 分子量 | 143.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人IQCH蛋白相关的文献信息及摘要概括：
1. **文献名称**：*Molecular cloning and characterization of human IQCH, a gene involved in spermatogenesis*
**作者**：Wang, Y., et al.
**摘要**：该研究首次克隆了人IQCH基因，发现其在睾丸组织中高表达。通过重组蛋白表达证实IQCH蛋白可通过IQ基序与钙调蛋白相互作用，推测其参与精子发生过程中的信号调控。
2. **文献名称**：*Expression and functional analysis of recombinant human IQCH protein in cellular models*
**作者**：Zhang, L., et al.
**摘要**：研究利用大肠杆菌系统成功表达并纯化重组人IQCH蛋白。体外实验表明该蛋白可与RNA结合蛋白相互作用，提示其在转录后调控及生殖细胞发育中的潜在作用。
3. **文献名称**：*IQCH regulates exosome-mediated cargo transport in Sertoli cells via interaction with CaM*
**作者**：Chen, R., et al.
**摘要**：通过构建IQCH重组质粒在支持细胞中的过表达模型，发现IQCH通过与钙调蛋白(CaM)的互作调控外泌体运输功能，进而影响血睾屏障的动态维持。
注：上述文献信息为综合领域知识生成的示例，实际文献可能需要通过专业数据库(如PubMed、Web of Science)检索验证。建议结合最新研究补充具体发表年份及期刊信息。
Recombinant human IQCH protein, a product of the *IQCH* gene, is engineered to study the molecular functions of IQ motif-containing protein H. The IQCH protein contains multiple IQ motifs, which are known to interact with calmodulin and other calcium-binding proteins, suggesting its involvement in calcium-mediated signaling pathways. It is predominantly expressed in testis and brain tissues, with emerging roles in spermatogenesis and neurological processes. Studies indicate that IQCH may act as an RNA-binding protein, participating in post-transcriptional regulation by interacting with specific mRNA targets during germ cell development.
The recombinant form is produced using expression systems like *E. coli* or mammalian cells, enabling functional and structural analyses. Research focuses on its potential association with male infertility, as IQCH knockout models in mice exhibit impaired sperm maturation and motility defects. Additionally, its interaction with components of the cell cytoskeleton and vesicle trafficking machinery highlights its broader regulatory functions.
Characterizing recombinant IQCH aids in elucidating its role in RNA metabolism, protein-protein interactions, and signaling cascades. These insights could advance diagnostic or therapeutic strategies for infertility and neurodegenerative disorders linked to IQ motif dysfunction. Current investigations also explore its splice variants and post-translational modifications to better understand its tissue-specific activities.
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