| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GHDC |
| Uniprot No | Q8N2G8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-530aa |
| 氨基酸序列 | MLLWPLLLLLLLLPTLALLRQQRSQDARLSWLAGLQHRVAWGALVWAATWQRRRLEQSTLHVHQSQQQALRWCLQGAQRPHCSLRRSTDISTFRNHLPLTKASQTQQEDSGEQPLPPTSNQDLGEASLQATLLGLAALNKAYPEVLAQGRTARVTLTSPWPRPLPWPGNTLGQVGTPGTKDPRALLLDALRSPGLRALEAGTAVELLDVFLGLETDGEELAGAIAAGNPGAPLRERAAELREALEQGPRGLALRLWPKLQVVVTLDAGGQAEAVAALGALWCQGLAFFSPAYAASGGVLGLNLQPEQPHGLYLLPPGAPFIELLPVKEGTQEEAASTLLLAEAQQGKEYELVLTDRASLTRCRLGDVVRVVGAYNQCPVVRFICRLDQTLSVRGEDIGEDLFSEALGRAVGQWAGAKLLDHGCVESSILDSSAGSAPHYEVFVALRGLRNLSEENRDKLDHCLQEASPRYKSLRFWGSVGPARVHLVGQGAFRALRAALAACPSSPFPPAMPRVLRHRHLAQCLQERVVS |
| 分子量 | 83.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GHDC蛋白的示例性参考文献(假设性内容，建议通过学术数据库验证准确性)：
1. **文献名称**：*Expression and Purification of Recombinant Human GHDC Protein in E. coli for Metabolic Studies*
**作者**：Zhang, Y. et al. (2021)
**摘要**：本研究利用大肠杆菌表达系统成功制备了重组人GHDC蛋白，并通过亲和层析纯化获得高纯度蛋白。实验证实GHDC在体外调控脂肪酸代谢途径，为研究其在代谢性疾病中的作用提供基础。
2. **文献名称**：*Structural Characterization of GHDC and Its Interaction with Insulin Signaling Pathways*
**作者**：Chen, L. et al. (2019)
**摘要**：通过哺乳动物细胞(HEK293)表达重组人GHDC蛋白，并解析其晶体结构。功能实验表明，GHDC通过与胰岛素受体结合增强下游信号传导，提示其在糖尿病治疗中的潜在价值。
3. **文献名称**：*GHDC Knockdown and Recombinant Protein Rescue in Cell Proliferation Assays*
**作者**：Wang, X. et al. (2020)
**摘要**：通过siRNA沉默细胞中内源性GHDC基因后，外源性添加重组GHDC蛋白可逆转细胞增殖抑制效应，证实其在细胞周期调控中的必要性。
4. **文献名称**：*GHDC in Cancer: Insights from Recombinant Protein-Based Models*
**作者**：Li, J. & Liu, M. (2022)
**摘要**：综述论文，汇总重组GHDC蛋白在肿瘤研究中的应用，强调其通过调控Wnt/β-catenin通路影响癌细胞迁移，并讨论规模化生产的挑战。
**注意事项**：
- 以上文献为模拟生成，GHDC蛋白可能为虚构缩写，建议核查**实际目标蛋白的全称**(如GH域蛋白或类似名称)。
- 实际文献需通过**PubMed、Google Scholar**等平台检索关键词(如“recombinant human GHDC protein”或“GHDC gene function”)。
- 若涉及拼写误差(如“GHDE”“GHDH”)，需调整关键词后重新搜索。
Recombinant human GHDC (Growth Hormone-Dependent C) protein is a genetically engineered form of the endogenous GHDC protein, designed for biomedical research and therapeutic applications. The native GHDC protein, also known as GH-dependent protein, is primarily associated with growth hormone (GH) signaling pathways. It functions as a mitochondrial protein involved in metabolic regulation, particularly in lipid metabolism and energy homeostasis. Studies suggest its role in modulating adipocyte differentiation, fatty acid oxidation, and insulin sensitivity, linking it to metabolic disorders like obesity and diabetes.
Recombinant GHDC is produced using expression systems such as E. coli or mammalian cell lines, ensuring high purity and bioactivity. It retains structural and functional characteristics of the native protein, including post-translational modifications when expressed in eukaryotic systems. This engineered protein is widely used to investigate GH-related signaling mechanisms, mitochondrial dysfunction, and metabolic disease pathologies. Its applications extend to drug screening, antibody development, and exploring therapeutic strategies for metabolic syndromes. Recent research also highlights its potential as a biomarker for GH-related disorders. Ongoing studies focus on elucidating its interactions with other metabolic regulators and its tissue-specific roles in energy balance.
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