| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GGTLA1 |
| Uniprot No | P36269 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-587aa |
| 氨基酸序列 | MARGYGATVSLVLLGLGLALAVIVLAVVLSRHQAPCGPQAFAHAAVAADSKVCSDIGRAILQQQGSPVDATIAALVCTSVVNPQSMGLGGGVIFTIYNVTTGKVEVINARETVPASHAPSLLDQCAQALPLGTGAQWIGVPGELRGYAEAHRRHGRLPWAQLFQPTIALLRGGHVVAPVLSRFLHNSILRPSLQASTLRQLFFNGTEPLRPQDPLPWPALATTLETVATEGVEVFYTGRLGQMLVEDIAKEGSQLTLQDLAKFQPEVVDALEVPLGDYTLYSPPPPAGGAILSFILNVLRGFNFSTESMARPEGRVNVYHHLVETLKFAKGQRWRLGDPRSHPKLQNASRDLLGETLAQLIRQQIDGRGDHQLSHYSLAEAWGHGTGTSHVSVLGEDGSAVAATSTINTPFGAMVYSPRTGIILNNELLDLCERCPRGSGTTPSPAVSGDRVGGAPGRCWPPVPGERSPSSMVPSILINKAQGSKLVIGGAGGELIISAVAQAIMSKLWLGFDLRAAIAAPILHVNSKGCVEYEPNFSQEVQRGLQDRGQNQTQRPFFLNVVQAVSQEGACVYAVSDLRKSGEAAGY |
| 分子量 | 88.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人GGTLA1蛋白的示例参考文献(注：以下文献为假设性示例，实际引用时请核实真实文献)：
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1. **文献名称**: *"Expression and Functional Characterization of Recombinant Human GGTLA1 in HEK293 Cells"*
**作者**: Wang, L. et al.
**摘要**: 本研究在大肠杆菌中表达并纯化了重组人GGTLA1蛋白，分析其谷氨酰转移酶活性。实验表明，该蛋白在体外可水解谷胱甘肽，并参与调节细胞氧化应激反应，为后续病理机制研究奠定基础。
2. **文献名称**: *"Structural Insights into GGTLA1: Implications for Substrate Specificity"*
**作者**: Kim, S. & Patel, R.
**摘要**: 通过X射线晶体学解析了重组人GGTLA1的三维结构，揭示其底物结合口袋的关键氨基酸残基。研究提出GGTLA1可能通过独特催化机制区别于其他GGT家族成员，为靶向药物设计提供依据。
3. **文献名称**: *"GGTLA1 Knockdown Attenuates Tumor Metastasis via ROS Pathway Modulation"*
**作者**: Chen, X. et al.
**摘要**: 利用重组GGTLA1蛋白验证其在肿瘤细胞中的功能，发现其通过调控ROS水平促进癌细胞侵袭。动物模型中，抑制GGTLA1显著降低转移能力，提示其作为癌症治疗靶点的潜力。
4. **文献名称**: *"Proteomic Analysis of GGTLA1-Interacting Proteins in Neurodegenerative Models"*
**作者**: Müller, T. et al.
**摘要**: 通过免疫共沉淀结合质谱技术，鉴定出与重组GGTLA1相互作用的神经退行性疾病相关蛋白(如α-synuclein)，表明GGTLA1可能通过蛋白相互作用参与帕金森病的病理进程。
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建议通过PubMed或Web of Science以关键词“GGTLA1”、“recombinant GGTLA1”或“gamma-glutamyltransferase-like activity 1”检索最新文献，或查阅相关综述以追踪研究进展。
Recombinant human GGTLA1 (Gamma-glutamyltransferase-like activity 1) protein is a key enzyme involved in glutathione metabolism and cellular redox regulation. Belonging to the gamma-glutamyltransferase (GGT) family, GGTLA1 catalyzes the hydrolysis of glutathione and transfers its gamma-glutamyl moiety to acceptor molecules, playing a critical role in maintaining intracellular antioxidant defense systems. Unlike canonical GGTs, GGTLA1 exhibits distinct structural and functional characteristics, including potential tissue-specific expression patterns and substrate preferences.
Produced through recombinant DNA technology, this protein is typically expressed in mammalian cell systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications and enzymatic activity. Its recombinant form enables researchers to study glutathione recycling, oxidative stress pathways, and its implications in diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions.
GGTLA1’s interaction with pro-inflammatory mediators and reactive oxygen species (ROS) highlights its dual role in both cytoprotection and pathological processes. Studies also explore its potential as a therapeutic target or biomarker for drug development. The recombinant protein serves as a vital tool for in vitro assays, inhibitor screening, and elucidating mechanisms underlying glutathione-dependent cellular responses.
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