| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM60A |
| Uniprot No | Q9NP50 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-221aa |
| 氨基酸序列 | MFGFHKPKMYRSIEGCCICRAKSSSSRFTDSKRYEKDFQSCFGLHETRSGDICNACVLLVKRWKKLPAGSKKNWNHVVDARAGPSLKTTLKPKKVKTLSGNRIKSNQISKLQKEFKRHNSDAHSTTSSASPAQSPCYSNQSDDGSDTEMASGSNRTPVFSFLDLTYWKRQKICCGIIYKGRFGEVLIDTHLFKPCCSNKKAAAEKPEEQGPEPLPISTQEW |
| 分子量 | 51.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM60A蛋白的示例参考文献(部分为假设性示例，实际文献需根据具体研究验证)：
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1. **文献名称**：*Structural and Functional Analysis of Human FAM60A in Transcriptional Repression*
**作者**：Söker, T. et al. (2020)
**摘要**：通过重组表达人FAM60A蛋白，解析其与SIN3A-HDAC复合物结合的晶体结构，证实其在基因转录抑制中通过招募组蛋白去乙酰化酶(HDAC)调控染色质修饰，影响细胞周期进程。
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2. **文献名称**：*FAM60A interacts with HP1 to regulate DNA damage response in cancer cells*
**作者**：Müller, J. et al. (2019)
**摘要**：利用重组FAM60A蛋白进行免疫共沉淀及体外结合实验，揭示其与异染色质蛋白HP1的相互作用，并发现敲低FAM60A导致DNA损伤修复缺陷，促进肿瘤细胞凋亡。
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3. **文献名称**：*Recombinant FAM60A facilitates stem cell pluripotency via epigenetic modulation*
**作者**：Chen, L. et al. (2021)
**摘要**：通过原核系统表达并纯化FAM60A蛋白，证明其在小鼠胚胎干细胞中维持多能性的作用依赖于对OCT4/SOX2靶基因启动子的表观遗传调控，与Polycomb抑制复合物存在协同效应。
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**注**：上述文献名称及内容为基于领域研究的概括性示例，具体文献需在PubMed、Web of Science等数据库按"FAM60A recombinant protein"或"FAM60A function"等关键词检索确认。实际研究可能聚焦于蛋白相互作用、结构分析或疾病机制。
**Background of Recombinant Human FAM60A Protein**
Recombinant human FAM60A (Family with sequence similarity 60 member A) is a protein encoded by the *FAM60A* gene, which is implicated in transcriptional regulation and chromatin remodeling. FAM60A interacts with the Sin3A-HDAC complex, a key epigenetic modifier involved in histone deacetylation and gene silencing. This association suggests its role in modulating chromatin structure, cell cycle progression, and differentiation.
Studies indicate that FAM60A is critical during embryonic development and cellular processes, including proliferation and apoptosis. Its dysfunction has been linked to developmental anomalies and cancer, where altered expression may influence tumorigenesis by dysregulating cell cycle checkpoints or epigenetic programs. Structurally, FAM60A contains conserved domains that facilitate protein-protein interactions, particularly with components of chromatin-modifying complexes.
The recombinant form of FAM60A is typically produced in *E. coli* or mammalian expression systems, enabling functional studies to dissect its molecular mechanisms. Purified recombinant FAM60A is used in assays exploring its biochemical activity, interactome, and role in epigenetic signaling pathways. Research on this protein contributes to understanding diseases associated with chromatin dysregulation, potentially aiding therapeutic strategies targeting epigenetic drivers in cancer or developmental disorders. Further studies are required to fully elucidate its context-dependent functions and clinical relevance.
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