| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM53B |
| Uniprot No | Q14153 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-305aa |
| 氨基酸序列 | MVMVLSESLSTRGADSIACGTFSRELHTPKKMSQGPTLFSCGIMENDRWRDLDRKCPLQIDQPSTSIWECLPEKDSSLWHREAVTACAVTSLIKDLSISDHNGNPSAPPSKRQCRSLSFSDEMSSCRTSWRPLGSKVWTPVEKRRCYSGGSVQRYSNGFSTMQRSSSFSLPSRANVLSSPCDQAGLHHRFGGQPCQGVPGSAPCGQAGDTWSPDLHPVGGGRLDLQRSLSCSHEQFSFVEYCPPSANSTPASTPELARRSSGLSRSRSQPCVLNDKKVGVKRRRPEEVQEQRPSLDLAKMAQIAP |
| 分子量 | 59.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇关于重组人FAM53B蛋白的参考文献示例(内容为模拟概括，建议通过学术数据库核实)：
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1. **文献名称**：*FAM53B interacts with PP2A to regulate Wnt/β-catenin signaling in colorectal cancer*
**作者**：Li X, Zhang Y, et al.
**摘要**：本研究阐明了重组人FAM53B蛋白通过与蛋白磷酸酶PP2A结合调控Wnt/β-catenin信号通路的机制，发现FAM53B过表达抑制结直肠癌细胞增殖，提示其作为肿瘤抑制因子的潜在作用。
2. **文献名称**：*Expression and purification of recombinant human FAM53B in mammalian cells for functional analysis*
**作者**：Wang H, Chen L, et al.
**摘要**：报道了一种在HEK293细胞中高效表达重组人FAM53B蛋白的方法，并优化了纯化步骤。研究验证了重组蛋白的稳定性及其在体外促进神经元分化的功能。
3. **文献名称**：*Structural insights into FAM53B’s role in oxidative stress response*
**作者**：Kim S, Park JH, et al.
**摘要**：通过晶体学分析重组人FAM53B的C端结构域，揭示其结合抗氧化蛋白Prx1的能力，表明FAM53B在细胞氧化应激调控中的关键作用。
4. **文献名称**：*FAM53B deficiency exacerbates cardiac hypertrophy via Hippo pathway dysregulation*
**作者**：Gao R, Liu T, et al.
**摘要**：利用重组FAM53B蛋白进行功能回补实验，证实其通过激活Hippo通路核心激酶LATS1抑制心肌肥厚，为心血管疾病治疗提供新靶点。
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**提示**：实际文献请通过PubMed、Google Scholar等平台以关键词“recombinant FAM53B”或“FAM53B function”检索，并关注近年研究更新。
Recombinant human FAM53B (Family with sequence similarity 53 member B) protein is a genetically engineered version of the naturally occurring protein encoded by the FAM53B gene, located on chromosome 17 (17q25.3). FAM53B, also designated as C17orf77. is a conserved but poorly characterized protein across vertebrates, suggesting potential roles in fundamental cellular processes. Structurally, it contains predicted coiled-coil domains and nuclear localization signals, implying involvement in protein interactions or subcellular trafficking.
Studies indicate FAM53B may regulate Wnt/β-catenin signaling, a critical pathway in development and disease, though its precise molecular mechanisms remain unclear. Recombinant FAM53B is typically produced in Escherichia coli or mammalian expression systems by cloning its cDNA into expression vectors, enabling purification via affinity tags. This tool facilitates functional studies, including protein-protein interaction mapping, enzymatic assays, and cellular localization experiments.
Emerging evidence links FAM53B to cancer progression and neurodevelopmental disorders. For instance, its dysregulation has been observed in glioblastoma and colorectal cancer, potentially influencing cell proliferation or apoptosis. In neurobiology, FAM53B deletion in mice causes behavioral abnormalities, suggesting roles in brain function. Further characterization of recombinant FAM53B could clarify its biological significance and therapeutic potential. Current research focuses on deciphering its interactome, post-translational modifications, and disease-associated mutations.
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