| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM54B |
| Uniprot No | Q9H019 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292aa |
| 氨基酸序列 | MSGMEATVTIPIWQNKPHGAARSVVRRIGTNLPLKPCARASFETLPNISDLCLRDVPPVPTLADIAWIAADEEETYARVRSDTRPLRHTWKPSPLIVMQRNASVPNLRGSEERLLALKKPALPALSRTTELQDELSHLRSQIAKIVAADAASASLTPDFLSPGSSNVSSPLPCFGSSFHSTTSFVISDITEETEVEVPELPSVPLLCSASPECCKPEHKAACSSSEEDDCVSLSKASSFADMMGILKDFHRMKQSQDLNRSLLKEEDPAVLISEVLRRKFALKEEDISRKGN |
| 分子量 | 58.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设情景构建的3篇关于重组人FAM54B蛋白的参考文献示例(请注意，以下内容为模拟生成，非真实存在的文献)：
---
1. **文献名称**：*Functional characterization of recombinant human FAM54B in cell cycle regulation*
**作者**：Li, X., Zhang, Y., & Wang, H.
**摘要**：本研究成功在大肠杆菌中表达并纯化了重组人FAM54B蛋白，发现其通过调控Cyclin D1和CDK4的表达影响细胞周期G1/S期转化，提示其在肿瘤发生中的潜在作用。
2. **文献名称**：*Structural analysis and interactome mapping of FAM54B via recombinant protein technology*
**作者**：Chen, Q., et al.
**摘要**：通过重组FAM54B的晶体结构解析和蛋白质互作组学分析，揭示了其包含典型的磷酸化位点，并与MAPK信号通路相关蛋白存在相互作用，可能参与炎症反应调控。
3. **文献名称**：*Recombinant FAM54B as a novel biomarker in hepatocellular carcinoma diagnosis*
**作者**：Gupta, R., et al.
**摘要**：利用哺乳动物细胞表达系统制备重组FAM54B，证实其在肝癌患者血清中显著高表达，且与肿瘤转移相关，具有作为诊断标志物的潜力。
---
**备注**：实际研究中FAM54B的相关文献较少，上述内容仅为基于领域知识的模拟。建议通过PubMed或Web of Science以“FAM54B”或“Family with sequence similarity 54 member B”为关键词检索最新文献。
**Background of Recombinant Human FAM54B Protein**
FAM54B (Family with Sequence Similarity 54 Member B) is a poorly characterized protein encoded by the *FAM54B* gene, located on human chromosome 8q22.1. Its biological functions remain largely unexplored, though preliminary studies suggest potential roles in cellular processes such as proliferation, apoptosis, and migration. Structurally, FAM54B contains conserved coiled-coil domains, which are often associated with protein-protein interactions, implying possible involvement in signaling pathways or cytoskeletal organization. The protein is expressed in various tissues, with higher levels observed in the brain, thyroid, and gastrointestinal tract, but its physiological significance in these contexts is unclear.
Recombinant human FAM54B protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling studies on its biochemical properties and interactions. Recent interest in FAM54B has emerged due to its potential association with diseases. For example, aberrant expression has been linked to cancers, including breast cancer, hepatocellular carcinoma, and gliomas, where it may influence tumor progression or drug resistance. However, mechanistic insights are lacking, and its exact role in oncogenesis or other pathologies remains speculative.
Current research focuses on clarifying FAM54B’s interactome, post-translational modifications (e.g., phosphorylation sites), and regulatory networks. The development of recombinant forms, antibodies, and knockout models aims to address gaps in understanding its cellular functions and therapeutic relevance. Despite limited data, FAM54B represents a novel target for exploring cancer biology and other diseases, warranting further investigation.
×
