| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COQ4 |
| Uniprot No | Q9Y3A0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-265aa |
| 氨基酸序列 | MATLLRPVLRRLCGLPGLQRPAAEMPLRARSDGAGPLYSHHLPTSPLQKALLAAGSAAMALYNPYRHDMVAVLGETTGHRTLKVLRDQMRRDPEGAQILQERPRISTSTLDLGKLQSLPEGSLGREYLRFLDVNRVSPDTRAPTRFVDDEELAYVIQRYREVHDMLHTLLGMPTNILGEIVVKWFEAVQTGLPMCILGAFFGPIRLGAQSLQVLVSELIPWAVQNGRRAPCVLNLYYERRWEQSLRALREELGITAPPMHVQGLA |
| 分子量 | 56.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COQ4蛋白的参考文献示例，按研究主题分类列举：
1. **标题**：*Functional Characterization of Recombinant Human COQ4 in Coenzyme Q10 Biosynthesis*
**作者**：Smith J, et al.
**摘要**：该研究通过体外实验验证重组人COQ4蛋白的酶活性，证实其与COQ3、COQ5等蛋白相互作用，协同促进辅酶Q10的生物合成。
2. **标题**：*High-Yield Expression and Purification of Recombinant Human COQ4 for Structural Analysis*
**作者**：Zhang L, Wang Y
**摘要**：开发了一种大肠杆菌表达系统，成功获得高纯度可溶性COQ4蛋白，为其结构解析(如X射线晶体学)奠定基础。
3. **标题**：*Rescue of COQ4-Deficient Cells by Recombinant COQ4 Protein in Mitochondrial Disorders*
**作者**：González MJ, et al.
**摘要**：揭示COQ4基因突变导致辅酶Q缺乏症，并通过导入重组COQ4蛋白恢复患者细胞线粒体功能，提示潜在的治疗价值。
4. **标题**：*COQ4 as a Scaffold Protein in CoQ-Synthome Assembly: Insights from Recombinant Interaction Studies*
**作者**：Kim T, Lee S
**摘要**：利用重组COQ4进行互作实验，发现其作为支架蛋白协调多酶复合体组装，维持辅酶Q合成效率。
**注**：以上文献为示例性质，实际研究中建议通过学术数据库(如PubMed、Web of Science)以关键词检索最新成果。
Recombinant human COQ4 protein is a key focus in mitochondrial research due to its essential role in coenzyme Q10 (CoQ10) biosynthesis. COQ4. encoded by the COQ4 gene, acts as a structural component of a multiprotein complex located in the mitochondrial inner membrane, facilitating the stabilization and activity of enzymes involved in CoQ10 production. CoQ10. a lipid-soluble electron carrier in the mitochondrial respiratory chain, also functions as a potent antioxidant. Deficiencies in CoQ10 biosynthesis are linked to mitochondrial disorders, including encephalomyopathy, cardiomyopathy, and renal dysfunction, often associated with COQ4 mutations. Recombinant COQ4 protein, typically expressed in systems like *E. coli* or mammalian cell cultures, enables detailed studies of its molecular interactions, enzymatic regulation, and pathological mechanisms. Its production allows researchers to analyze mutations affecting protein stability or complex assembly, providing insights into disease pathogenesis. Additionally, recombinant COQ4 serves as a tool for screening therapeutic compounds aimed at restoring CoQ10 levels in genetic or age-related deficiencies. Current research explores its potential in gene therapy and pharmacological chaperone-based treatments. The development of high-purity recombinant COQ4 remains critical for advancing diagnostic assays, functional studies, and targeted therapies for mitochondrial diseases linked to CoQ10 dysfunction.
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