| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COPZ2 |
| Uniprot No | Q9P299 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-210aa |
| 氨基酸序列 | MQRPEAWPRP HPGEGAAAAQ AGGPAPPARA GEPSGLRLQE PSLYTIKAVF ILDNDGRRLL AKYYDDTFPS MKEQMVFEKN VFNKTSRTES EIAFFGGMTI VYKNSIDLFL YVVGSSYENE LMLMSVLTCL FESLNHMLRK NVEKRWLLEN MDGAFLVLDE IVDGGVILES DPQQVIQKVN FRADDGGLTE QSVAQVLQSA KEQIKWSLLK |
| 分子量 | 23.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COPZ2蛋白的3篇虚拟参考文献示例(仅供参考，非真实文献)：
1. **文献名称**：Structural Insights into Human COPZ2 Protein Function in COPI Vesicle Assembly
**作者**：Zhang L, et al.
**摘要**：本研究通过重组表达人COPZ2蛋白，利用X射线晶体学解析其与COPI复合体亚基的相互作用结构，揭示了COPZ2在囊泡运输中调控货物分选的分子机制。
2. **文献名称**：Recombinant Human COPZ2 Expression and Its Role in Golgi-to-ER Retrograde Transport
**作者**：Kimura T, et al.
**摘要**：开发了人COPZ2蛋白的原核重组表达系统，并通过体外实验证明其在逆向运输中通过结合KDEL受体调控内质网驻留蛋白的回收功能。
3. **文献名称**：COPZ2 Knockdown and Overexpression Reveal Its Dual Role in Cancer Cell Proliferation
**作者**：Chen H, et al.
**摘要**：利用重组人COPZ2蛋白进行功能研究，发现其表达水平异常通过干扰脂代谢通路影响肝癌细胞增殖，提示其作为潜在癌症治疗靶点。
注：以上文献为基于领域知识的模拟示例，实际研究中请通过PubMed或Web of Science等平台检索真实文献。
Recombinant human COPZ2 protein is a lab-engineered version of the cytoplasmic coatomer complex subunit ζ2 (COPZ2), a conserved component of the COPI (coatomer protein complex I) vesicle transport system. COPI vesicles mediate retrograde trafficking between the Golgi apparatus and the endoplasmic reticulum (ER), maintaining organelle integrity, cargo sorting, and intracellular lipid homeostasis. The COPZ2 subunit, along with COPZ1. forms the ζ-COPI subcomplex, which plays a critical role in coatomer assembly and vesicle budding. Unlike ubiquitously expressed COPZ1. COPZ2 exhibits tissue-specific expression patterns and may regulate specialized trafficking pathways in certain cell types, though its distinct functional roles remain less characterized.
Recombinant COPZ2 is typically produced in bacterial or eukaryotic expression systems, enabling studies on COPI dynamics, vesicle formation mechanisms, and interactions with cargo receptors or regulatory proteins. Its applications span structural biology (e.g., crystallography to resolve coatomer architecture), biochemical assays (e.g., GTPase activity modulation), and disease research, particularly in cancers and metabolic disorders linked to disrupted intracellular trafficking. Investigating COPZ2-specific functions may reveal novel insights into COPI diversity and tissue-specific vesicular transport pathways.
×
