| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COPS5 |
| Uniprot No | Q92905 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-344aa |
| 氨基酸序列 | MAASGSGMAQKTWELANNMQEAQSIDEIYKYDKKQQQEILAAKPWTKDHHYFKYCKISALALLKMVMHARSGGNLEVMGLMLGKVDGETMIIMDSFALPVEGTETRVNAQAAAYEYMAAYIENAKQVGRLENAIGWYHSHPGYGCWLSGIDVSTQMLNQQFQEPFVAVVIDPTRTISAGKVNLGAFRTYPKGYKPPDEGPSEYQTIPLNKIEDFGVHCKQYYALEVSYFKSSLDRKLLELLWNKYWVNTLSSSSLLTNADYTTGQVFDLSEKLEQSEAQLGRGSFMLGLETHDRKSEDKLAKATRDSCKTTIEAIHGLMSQVIKDKLFNQINIS |
| 分子量 | 62.48 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COPS5蛋白研究的3篇示例参考文献(格式与内容为模拟，实际文献需通过学术数据库查询)：
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1. **文献名称**: *"Recombinant human COPS5 regulates the ubiquitin-proteasome system via modulating cullin-RING ligase activity"*
**作者**: Li et al.
**摘要**: 研究通过大肠杆菌表达系统纯化了重组人COPS5蛋白，证实其通过去NEDD化作用调控CRLs(Cullin-RING ligases)的活性，进而影响细胞周期蛋白的稳定性，揭示了其在肿瘤发生中的潜在作用。
2. **文献名称**: *"Structural insights into the interaction of COPS5 with the COP9 signalosome complex"*
**作者**: Wang Y, Zhang H.
**摘要**: 利用X射线晶体学解析了重组人COPS5与COP9信号复合体其他亚基的结合结构，阐明了其JAMM结构域在介导复合体组装中的关键作用，为靶向COPS5的药物设计提供了结构基础。
3. **文献名称**: *"Functional characterization of recombinant COPS5 in DNA damage response"*
**作者**: Kumar S et al.
**摘要**: 通过哺乳动物细胞表达重组COPS5.证明其在DNA损伤修复中通过调节p53蛋白的稳定性影响细胞凋亡，提示COPS5可能是癌症治疗的潜在靶点。
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**备注**：以上文献信息为示例，实际引用需查阅PubMed、Web of Science等平台获取真实研究。如需具体文献推荐，请提供更详细的研究方向(如疾病模型、相互作用蛋白等)。
**Background of Recombinant Human COPS5 Protein**
COPS5 (COP9 Signalosome Subunit 5), also known as CSN5 or JAB1. is a critical component of the COP9 signalosome (CSN), an evolutionarily conserved multi-protein complex involved in diverse cellular processes, including protein degradation, DNA repair, and signal transduction. The CSN complex regulates the ubiquitin-proteasome system by modulating cullin-RING ligase (CRL) activity through deneddylation of cullin proteins. COPS5. as the catalytic subunit, harbors a JAMM/MPN+ metalloprotease domain responsible for enzymatic activity.
Recombinant human COPS5 protein is engineered using biotechnological methods (e.g., *E. coli* or mammalian expression systems) to enable high-purity, functional studies. Its recombinant form allows researchers to explore molecular mechanisms underlying its role in cell cycle regulation, apoptosis, and responses to DNA damage. Dysregulation of COPS5 is linked to cancer progression, neurodegenerative disorders, and immune diseases due to its interaction with key signaling pathways (e.g., NF-κB, p53. and HIF-1α). Studies leveraging recombinant COPS5 have advanced drug discovery, particularly in targeting CSN-associated pathologies. This protein serves as a vital tool for elucidating CSN-mediated post-translational modifications and their implications in health and disease.
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