| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COPZ1 |
| Uniprot No | P61923 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-177aa |
| 氨基酸序列 | MEALILEPSLYTVKAILILDNDGDRLFAKYYDDTYPSVKEQKAFEKNIFNKTHRTDSEIALLEGLTVVYKSSIDLYFYVIGSSYENELMLMAVLNCLFDSLSQMLRKNVEKRALLENMEGLFLAVDEIVDGGVILESDPQQVVHRVALRGEDVPLTEQTVSQVLQSAKEQIKWSLLR |
| 分子量 | 46.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COPZ1蛋白的3篇代表性文献示例(内容基于领域相关研究总结，非真实文献)：
1. **"Structural insights into human COPZ1 protein and its interaction with COPI machinery"**
- 作者：Li, X. et al.
- 摘要：解析了重组人COPZ1蛋白的晶体结构，揭示了其保守的β-propeller结构域，并通过体外结合实验证明COPZ1与COPα亚基直接互作，参与调控COPI囊泡的形成。
2. **"COPZ1 depletion disrupts Golgi-ER trafficking and lipid homeostasis"**
- 作者：Wang, Y. et al.
- 摘要：利用重组COPZ1蛋白进行功能回补实验，发现COPZ1通过调控COPI复合体介导的逆向运输，影响细胞内脂滴形成和胆固醇代谢平衡。
3. **"Recombinant human COPZ1 as a tool for studying coatomer assembly"**
- 作者：Smith, J.R. & Beck, R.
- 摘要：开发了大肠杆菌表达的重组人COPZ1蛋白纯化方法，并通过体外重组实验证实COPZ1是COPI囊泡组装的关键因子，其功能依赖与Arf1 GTP酶的结合。
(注：实际研究中可结合关键词 **COPZ1、COPI coatomer、vesicular transport、protein trafficking** 在PubMed或Web of Science检索近期文献。)
**Background of Recombinant Human COPZ1 Protein**
Recombinant human COPZ1 protein is a key component of the coatomer protein complex I (COPI), which plays a central role in intracellular vesicular trafficking. COPZ1 (Coatomer Protein Complex Subunit Zeta 1) is involved in the formation of COPI-coated vesicles, facilitating retrograde transport of proteins and lipids from the Golgi apparatus to the endoplasmic reticulum (ER). This process ensures proper sorting, recycling, and maintenance of cellular organelle integrity.
Structurally, COPZ1 contains a conserved WD40 repeat domain, enabling interactions with other coatomer subunits and cargo receptors. Its function is critical for regulating membrane dynamics, secretory pathways, and autophagy. Dysregulation of COPZ1 has been implicated in pathologies such as cancer and neurodegenerative disorders, highlighting its importance in cellular homeostasis.
Recombinant COPZ1 is typically produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to ensure high purity and functionality. It serves as a vital tool for *in vitro* studies, including protein-protein interaction assays, structural analyses, and mechanistic investigations of COPI-mediated trafficking. Researchers also utilize recombinant COPZ1 to explore therapeutic targets by modulating vesicle formation or cargo sorting in disease models.
Overall, recombinant COPZ1 provides a versatile platform for elucidating the molecular mechanisms of intracellular transport and developing strategies to address trafficking-related disorders.
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