| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COQ2 |
| Uniprot No | Q96H96 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-371aa |
| 氨基酸序列 | MLGSRAAGFARGLRALALAWLPGWRGRSFALARAAGAPHGGDLQPPACPEPRGRQLSLSAAAVVDSAPRPLQPYLRLMRLDKPIGTWLLYLPCTWSIGLAAEPGCFPDWYMLSLFGTGAILMRGAGCTINDMWDQDYDKKVTRTANRPIAAGDISTFQSFVFLGGQLTLALGVLLCLNYYSIALGAGSLLLVITYPLMKRISYWPQLALGLTFNWGALLGWSAIKGSCDPSVCLPLYFSGVMWTLIYDTIYAHQDKRDDVLIGLKSTALRFGENTKPWLSGFSVAMLGALSLVGVNSGQTAPYYAALGAVGAHLTHQIYTLDIHRPEDCWNKFISNRTLGLIVFLGIVLGNLWKEKKTDKTKKGIENKIEN |
| 分子量 | 66.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3-4条关于重组人COQ2蛋白的参考文献(示例为模拟虚构条目，仅作参考格式示范)：
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1. **文献名称**: "Functional Expression and Characterization of Recombinant Human COQ2 in Escherichia coli"
**作者**: Yamada K, et al.
**摘要**: 研究报道了在大肠杆菌中高效表达可溶性的重组人COQ2蛋白，通过优化密码子和纯化工艺获得高纯度蛋白。酶活分析证实其具有催化对羟基苯甲酸酯聚异戊二烯化反应的功能，为后续辅酶Q10合成机制研究提供基础。
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2. **文献名称**: "COQ2 Mutations Impair Enzymatic Activity in Mitochondrial Disorders"
**作者**: Johnson M, et al.
**摘要**: 通过构建重组人COQ2突变体(如R387H)，发现特定突变显著降低酶活性，导致辅酶Q10合成缺陷。该研究揭示了COQ2基因突变与线粒体脑肌病及多系统疾病的分子关联。
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3. **文献名称**: "Structural Insights into Human COQ2 by Cryo-EM and Its Role in Antioxidant Defense"
**作者**: Chen L, et al.
**摘要**: 利用冷冻电镜解析重组人COQ2蛋白的三维结构，发现其催化口袋的保守区域对底物结合至关重要。功能实验表明COQ2表达上调可增强细胞抗氧化能力，为相关代谢疾病治疗提供靶点。
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4. **文献名称**: "High-Yield Production of Recombinant COQ2 in Mammalian Cells for Drug Screening"
**作者**: Smith J, et al.
**摘要**: 开发基于HEK293细胞的重组人COQ2稳定表达系统，实现大规模制备功能性蛋白。结合高通量筛选技术，发现小分子化合物可调控COQ2活性，为辅酶Q10缺乏症药物研发提供平台。
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注：以上文献为模拟内容，实际研究中请通过PubMed、Web of Science等数据库检索真实文献。
**Background of Recombinant Human COQ2 Protein**
The human COQ2 gene encodes a mitochondrial enzyme, para-hydroxybenzoate polyprenyltransferase, which plays a critical role in coenzyme Q10 (CoQ10) biosynthesis. CoQ10. a lipid-soluble quinone, is essential for mitochondrial electron transport and cellular energy production. COQ2 catalyzes the condensation of the aromatic precursor 4-hydroxybenzoate with a polyprenyl diphosphate chain, forming the intermediate 3-polyprenyl-4-hydroxybenzoate. This step determines the length of the CoQ10 sidechain, influencing its biological activity.
Mutations in COQ2 are linked to primary CoQ10 deficiency, a rare mitochondrial disorder associated with encephalopathy, nephropathy, and myopathy. Recombinant human COQ2 protein is produced using biotechnological platforms (e.g., bacterial or mammalian expression systems) to study its enzymatic function, structure, and interactions. Such recombinant proteins enable mechanistic insights into disease-causing variants and facilitate drug screening for CoQ10 deficiency therapies.
Additionally, recombinant COQ2 holds potential for therapeutic applications, including enzyme replacement strategies or gene therapy. Its study also contributes to understanding mitochondrial dysfunction in aging and neurodegenerative diseases. Standardized production of active recombinant COQ2 remains challenging due to its membrane-associated nature and requirement for proper folding. Nonetheless, advances in protein engineering continue to enhance its utility in biomedical research and translational medicine.
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